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A possible role of transglutaminase 2 in the nucleus of INS-1E and of cells of human pancreatic islets

Sileno, Sara
D'Oria, Valentina
Stucchi, Riccardo
Alessio, Massimo
Petrini, Stefania
Bonetto, Valentina
Bertuzzi, Federico
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Published in Journal of Proteomics. 2014, vol. 96, p. 314-327
Abstract Transglutaminase 2 (TG2) is a multifunctional protein with Ca(2+)-dependent transamidating and G protein activity. Previously we reported that the role of TG2 in insulin secretion may involve cytoplasmic actin remodeling and a regulative action on other proteins during granule movement. The aim of this study was to gain a better insight into the role of TG2 transamidating activity in mitochondria and in the nucleus of INS-1E rat insulinoma cell line (INS-1E) during insulin secretion. To this end we labeled INS-1E with an artificial donor (biotinylated peptide), in basal condition and after stimulus with glucose for 2, 5, and 8min. Biotinylated proteins of the nuclear/mitochondrial-enriched fraction were analyzed using two-dimensional electrophoresis and mass spectrometry. Many mitochondrial proteins involved in Ca(2+) homeostasis (e.g. voltage-dependent anion-selective channel protein, prohibitin and different ATP synthase subunits) and many nuclear proteins involved in gene regulation (e.g. histone H3, barrier to autointegration factor and various heterogeneous nuclear ribonucleoprotein) were identified among a number of transamidating substrates of TG2 in INS-1E. The combined results provide evidence that a temporal link exists between glucose-stimulation, first phase insulin secretion and the action of TG on histone H3 both in INS-1E and human pancreatic islets.
Keywords AnimalsCalcium/metabolismCell Line, TumorCell Nucleus/enzymologyGlucose/pharmacologyHumansInsulin/secretionInsulin-Secreting Cells/cytology/enzymologyRatsSweetening Agents/pharmacologyTranscription, Genetic/drug effects/physiologyTransglutaminases/genetics/metabolism
PMID: 24291354
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Research group Mitochondries et métabolisme énergétique (671)
Swiss National Science Foundation: 310030B_135704
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SILENO, Sara et al. A possible role of transglutaminase 2 in the nucleus of INS-1E and of cells of human pancreatic islets. In: Journal of proteomics, 2014, vol. 96, p. 314-327. doi: 10.1016/j.jprot.2013.11.011 https://archive-ouverte.unige.ch/unige:46794

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Deposited on : 2015-02-18

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