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A portable microfluidic-based biophotonic sensor for extracellular H2O2 measurements

Koman, Volodymyr
Suárez, Guillaume
Santschi, Christian
Cadarso, V.J.
Brugger, J
Martin, O.J.F.
Publication San Francisco, California, 2013
Collection Proc. SPIE 8572, Advanced Biomedical and Clinical Diagnostic Systems XI
Abstract In this work a portable analytical biosensor for real-time extracellular monitoring of released hydrogen peroxide (H2O2 ) is presented. The biosensor is based on the optical detection of the cytochrome c (cyt c) oxidation state. The setup consists of an integrated microscope combined with a compact spectrometer. The light being absorbed by cyt c is enhanced via multiscattering produced by random aggregates of polystyrene beads in a cross-linked cyt c matrix. Using ink-jet printing technique, the sensing elements, namely cyt c loaded polystyrene aggregates, are fabricated with high reliability in terms of repeatability of size and sensitivity. Additionally, the sensing elements are enclosed in a microfluidic channel assuring a fast and efficient analytes delivery. As an example, the effect of trace concentrations of functionalized cadmium selenide/zinc sulfide (CdSe/ZnS) core shell quantum dots on the green algae Chlamydomonas reinhardtii is investigated, showing extracellular H2O2 release with different production rates over a period of 1 hour. In conclusion, the presented portable biosensor enables the highly sensitive and non-invasive real-time monitoring of the cell metabolism of C. reinhardtii. © (2013) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Keywords Portable biosensorInk-jetMicrofluidicsOxidative stressReactive oxygen speciesQuantum dots
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KOMAN, Volodymyr et al. A portable microfluidic-based biophotonic sensor for extracellular H2O2 measurements. San Francisco, California : [s.n.], 2013. (Proc. SPIE 8572, Advanced Biomedical and Clinical Diagnostic Systems XI) https://archive-ouverte.unige.ch/unige:46713

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