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A recombinant yeast strain as a short term bioassay to assess estrogen-like activity of xenobiotics

Pinto, B.
Reali, D.
Published in Annali di igiene. 2004, vol. 16, no. 4, p. 579-85
Abstract The study investigated the responsiveness of a recombinant Saccharomyces cerevisiae yeast strain to screen estrogen-like chemicals in order to use it as a first level short-term bioassay for environmental preventive medicine purposes. We used the yeast strain RMY326 ER-ERE, containing the human estrogen receptor alpha and a reporter gene lacZ encoding the enzyme b-galactosidase. We tested the natural steroid hormone 17beta-estradiol, diethylstilbestrol, colchicine, some phytoestrogens and pesticides, and organochlorine insecticides. The relative inductive efficiency (RIE) and EC50 values confirmed the sensitivity and specificity of the yeast strain. A statistically significant reproducibility of results in repeated experiments was assessed.
Keywords Biological Assay/methodsDNA, FungalDNA, RecombinantEstrogen Receptor alpha/geneticsEstrogens/physiologySaccharomyces cerevisiae/geneticsTime FactorsXenobiotics/pharmacology
PMID: 15366516
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PINTO, B., PICARD, Didier, REALI, D. A recombinant yeast strain as a short term bioassay to assess estrogen-like activity of xenobiotics. In: Annali di igiene, 2004, vol. 16, n° 4, p. 579-85. https://archive-ouverte.unige.ch/unige:4660

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Deposited on : 2009-12-11

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