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Scientific article
English

A recombinant yeast strain as a short term bioassay to assess estrogen-like activity of xenobiotics

Contributors

Pinto, B.; Picard, Didier; Reali, D.

Published in

Annali di igiene medicina preventiva e di comunità, vol. 16, no. 4, p. 579-585

Publication date

2004

Abstract

The study investigated the responsiveness of a recombinant Saccharomyces cerevisiae yeast strain to screen estrogen-like chemicals in order to use it as a first level short-term bioassay for environmental preventive medicine purposes. We used the yeast strain RMY326 ER-ERE, containing the human estrogen receptor alpha and a reporter gene lacZ encoding the enzyme b-galactosidase. We tested the natural steroid hormone 17beta-estradiol, diethylstilbestrol, colchicine, some phytoestrogens and pesticides, and organochlorine insecticides. The relative inductive efficiency (RIE) and EC50 values confirmed the sensitivity and specificity of the yeast strain. A statistically significant reproducibility of results in repeated experiments was assessed.

Keywords

  • Biological Assay/methods
  • DNA, Fungal
  • DNA, Recombinant
  • Estrogen Receptor alpha/genetics
  • Estrogens/physiology
  • Saccharomyces cerevisiae/genetics
  • Time Factors
  • Xenobiotics/pharmacology

UNIGE affiliation entities

Citation (ISO format)

PINTO, B., PICARD, Didier, REALI, D. A recombinant yeast strain as a short term bioassay to assess estrogen-like activity of xenobiotics. In: Annali di igiene medicina preventiva e di comunità, 2004, vol. 16, n° 4, p. 579–585.

Main files (1)

Article (Published version)

Identifiers

Journal ISSN

1120-9135
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Technical informations

Creation

11/12/2009 11:15:00

First validation

11/12/2009 11:15:00

Update

14/03/2023 15:19:37

Status update

14/03/2023 15:19:37

Last indexation

29/10/2024 12:47:58
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