en
Scientific article
English

The direct proliferative stimulus of dehydroepiandrosterone on MCF7 breast cancer cells is potentiated by overexpression of aromatase

Published inMolecular and cellular endocrinology, vol. 184, no. 1-2, p. 163-171
Publication date2001
Abstract

In women after menopause aromatization of adrenal androgens represents the main source of estrogens, which may promote the development of hormone-dependent breast tumor. Several studies have attempted to determine the cell type within carcinomas that is responsible for 'in situ' estrogen biosynthesis and whether the amount produced may sustain relevant biological effects. Here we show P450arom mRNA and protein expression together with immunocytochemical localization of aromatase in the epithelial MCF7 breast cancer cell line. Moreover, we demonstrate that the enhanced aromatization of dehydroepiandrosterone in aromatase transfected MCF7 cells confers biological advantages such as proliferative stimulation similar to that induced by estradiol. Our results suggest that aromatase inhibitors may be particularly effective in the treatment of hormone-dependent breast cancer disease in postmenopausal women.

Keywords
  • Aromatase/genetics/metabolism/pharmacology
  • Breast Neoplasms/enzymology/pathology
  • Cell Division/drug effects
  • Dehydroepiandrosterone/metabolism/pharmacology
  • Drug Synergism
  • Estradiol/biosynthesis/secretion
  • Estrogen Receptor alpha
  • Female
  • Humans
  • Immunohistochemistry
  • RNA, Messenger/analysis
  • Receptors, Estrogen/metabolism
  • Transfection
  • Tumor Cells, Cultured
Citation (ISO format)
MAGGIOLINI, M. et al. The direct proliferative stimulus of dehydroepiandrosterone on MCF7 breast cancer cells is potentiated by overexpression of aromatase. In: Molecular and cellular endocrinology, 2001, vol. 184, n° 1-2, p. 163–171. doi: 10.1016/S0303-7207(01)00563-9
Main files (1)
Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal0303-7207
517views
0downloads

Technical informations

Creation12/08/2009 12:34:00 PM
First validation12/08/2009 12:34:00 PM
Update time03/14/2023 3:19:36 PM
Status update03/14/2023 3:19:36 PM
Last indexation01/15/2024 7:23:25 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack