en
Scientific article
Open access
English

TROD: T7 RNAi oligo designer

Published inNucleic acids research, vol. 32, p. W121-123
Publication date2004
Abstract

We have developed T7 RNAi Oligo Designer (TROD), a web application for RNA interference studies. TROD greatly facilitates the design of oligodeoxynucleotide sequences for the in vitro production of siRNA duplexes with T7 RNA polymerase. Given a query cDNA sequence, the program scans for appropriate target sequences based on the constraints of the T7 RNA polymerase method and published criteria for RNA interference with siRNAs. The output is an ordered and prioritized list of ready-to-order DNA oligonucleotide sequences, with links to perform a BLAST search to ascertain target sequence specificity. The TROD web service is available at http://www.cellbio.unige.ch/RNAi.html.

Keywords
  • DNA-Directed RNA Polymerases/metabolism
  • Internet
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides/biosynthesis/chemistry
  • RNA Interference
  • RNA, Small Interfering/biosynthesis/chemistry
  • Software
  • Viral Proteins
Citation (ISO format)
DUDEK, Peter, PICARD, Didier. TROD: T7 RNAi oligo designer. In: Nucleic acids research, 2004, vol. 32, p. W121–123. doi: 10.1093/nar/gkh360
Main files (1)
Article (Published version)
accessLevelPublic
Identifiers
ISSN of the journal0305-1048
593views
319downloads

Technical informations

Creation12/04/2009 11:20:00 AM
First validation12/04/2009 11:20:00 AM
Update time03/14/2023 3:19:28 PM
Status update03/14/2023 3:19:28 PM
Last indexation05/02/2024 11:25:54 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack