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Scientific article
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English

TROD: T7 RNAi oligo designer

Contributors

Dudek, Peter; Picard, Didier

Published in

Nucleic acids research, vol. 32, p. W121-123

Publication date

2004

Abstract

We have developed T7 RNAi Oligo Designer (TROD), a web application for RNA interference studies. TROD greatly facilitates the design of oligodeoxynucleotide sequences for the in vitro production of siRNA duplexes with T7 RNA polymerase. Given a query cDNA sequence, the program scans for appropriate target sequences based on the constraints of the T7 RNA polymerase method and published criteria for RNA interference with siRNAs. The output is an ordered and prioritized list of ready-to-order DNA oligonucleotide sequences, with links to perform a BLAST search to ascertain target sequence specificity. The TROD web service is available at http://www.cellbio.unige.ch/RNAi.html.

Keywords

  • DNA-Directed RNA Polymerases/metabolism
  • Internet
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides/biosynthesis/chemistry
  • RNA Interference
  • RNA, Small Interfering/biosynthesis/chemistry
  • Software
  • Viral Proteins

UNIGE affiliation entities

Citation (ISO format)

DUDEK, Peter, PICARD, Didier. TROD: T7 RNAi oligo designer. In: Nucleic acids research, 2004, vol. 32, p. W121–123. doi: 10.1093/nar/gkh360

Main files (1)

Article (Published version)

Identifiers

Journal ISSN

0305-1048
695views
446downloads

Technical informations

Creation

04/12/2009 11:20:00

First validation

04/12/2009 11:20:00

Update

14/03/2023 15:19:28

Status update

14/03/2023 15:19:28

Last indexation

29/10/2024 12:46:58
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