en
Scientific article
English

Bioactive steroids as contaminants of the common carbon source galactose

Published inFEMS microbiology letters, vol. 159, no. 2, p. 167-171
Publication date1998
Abstract

Most inducible expression vectors for the budding yeast Saccharomyces cerevisiae are based on galactose-inducible promoters. Yeast has been increasingly used to study vertebrate steroid receptors because of its powerful genetics. In principle, both regulatory systems are compatible and can be combined in the same strain. However, we found that commercial galactose can be contaminated by bioactive estrogen and progesterone at concentrations that are sufficient to fully activate their cognate receptors. Since steroids can elicit biological responses in pathogenic fungi and possibly other microorganisms, such contaminants in a commonly used fermentable carbon source may need to be screened for.

Keywords
  • Drug Contamination
  • Estrogens/analysis
  • Galactose/analysis/pharmacology
  • Progesterone/analysis
  • Receptors, Steroid/drug effects
Citation (ISO format)
LIU, J W., PICARD, Didier. Bioactive steroids as contaminants of the common carbon source galactose. In: FEMS microbiology letters, 1998, vol. 159, n° 2, p. 167–171. doi: 10.1111/j.1574-6968.1998.tb12856.x
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Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal0378-1097
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