Scientific article

Infections ostéo-articulaires septiques à culture négative chez l'enfant : avez-vous pensé à Kingella kingae ?

Published inRevue médicale suisse, vol. 5, no. 224, p. 2235-2239
Publication date2009

Kingella kingae is an emerging pathogen that is recognized as a causative agent of septic arthritis and osteomyelitis, primarily in infants and children. The bacterium is best detected by rapid inoculation in blood culture systems or by real-time PCR assays. Pathogenesis of the agent was linked recently to the production of a potent cytotoxin, known as RTX, which is toxic to a variety of human cell types. The locus encoding the RTX toxin is thought to be a putative virulence factor, and is, apparently, essential for inducing cytotoxic effects on respiratory epithelial, synovial and macrophage-like cells. Herein, we describe a novel real-time PCR assay that targets the RTX toxin gene. The assay exhibited a sensitivity of 30 c.f.u., which is 10-fold more sensitive than a previously published semi-nested broad-range 16S rRNA gene PCR, and showed no crossreactivity with several related species and common osteoarticular pathogens. Its clinical impact is illustrated by three pediatric cases.

  • Bone Diseases, Infectious/diagnosis
  • Child, Preschool
  • Female
  • Humans
  • Infant
  • Kingella kingae
  • Male
  • Neisseriaceae Infections/diagnosis
Citation (ISO format)
CHERKAOUI, Abdessalam et al. Infections ostéo-articulaires septiques à culture négative chez l’enfant : avez-vous pensé à Kingella kingae ? In: Revue médicale suisse, 2009, vol. 5, n° 224, p. 2235–2239.
Main files (1)
Article (Published version)
ISSN of the journal1660-9379

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