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Infections ostéo-articulaires septiques à culture négative chez l'enfant : avez-vous pensé à Kingella kingae ?

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Published in Revue médicale suisse. 2009, vol. 5, no. 224, p. 2235-9
Abstract Kingella kingae is an emerging pathogen that is recognized as a causative agent of septic arthritis and osteomyelitis, primarily in infants and children. The bacterium is best detected by rapid inoculation in blood culture systems or by real-time PCR assays. Pathogenesis of the agent was linked recently to the production of a potent cytotoxin, known as RTX, which is toxic to a variety of human cell types. The locus encoding the RTX toxin is thought to be a putative virulence factor, and is, apparently, essential for inducing cytotoxic effects on respiratory epithelial, synovial and macrophage-like cells. Herein, we describe a novel real-time PCR assay that targets the RTX toxin gene. The assay exhibited a sensitivity of 30 c.f.u., which is 10-fold more sensitive than a previously published semi-nested broad-range 16S rRNA gene PCR, and showed no crossreactivity with several related species and common osteoarticular pathogens. Its clinical impact is illustrated by three pediatric cases.
Keywords Bone Diseases, Infectious/diagnosisChild, PreschoolFemaleHumansInfantKingella kingaeMaleNeisseriaceae Infections/diagnosis
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PMID: 19994673
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Other version: http://rms.medhyg.ch/numero-224-page-2235.htm
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CHERKAOUI, Abdessalam et al. Infections ostéo-articulaires septiques à culture négative chez l'enfant : avez-vous pensé à Kingella kingae ?. In: Revue médicale suisse, 2009, vol. 5, n° 224, p. 2235-9. https://archive-ouverte.unige.ch/unige:43837

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Deposited on : 2014-12-18

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