The main goal of the Swiss-Prot section of the UniProt Knowledgebase (UniProtKB) is to ensure the presence of high-quality annotation for representative members of most protein families, and Swiss-Prot curators mainly concentrate their efforts on proteins from model organisms. Dictyostelium discoideum is one of the few organisms selected by the National Institutes of Health (NIH) as suitable for functional analysis of sequenced genes, and researchers working on this model benefit from the existence of dictyBase, the official and inestimable resource for Dictyostelium discoideum genome annotation. Yet, annotation of the Dictyostelium discoideum proteome was absent from UniProt's priority list until the launch of a collaboration between dictyBase and UniProtKB. This chapter reviews the Swiss-Prot annotation process by taking examples from the recently annotated part of the Dictyostelium discoideum proteome, and shows how fruitful a collaboration can be between a model organism database and the generalist database UniProtKB.