13-lipoxygenases (13-LOXs) initiate the first enzymatic reaction in jasmonate synthesis which is essential for the activation of defence against herbivores. Jasmonates are wound- inducible compounds that accumulate in a wide time frame from seconds to hours after tissue damage. Jasmonate production begins in plastids and, in Arabidopsis thaliana, four 13-LOXs (LOX2, LOX3, LOX4 and LOX6) incorporate molecular oxygen on the C13 of the α-linolenic acid. Among the four 13-LOXs, LOX2 has been the most widely studied while LOX3, LOX4 and LOX6 remain poorly described. In the present work, we found that each 13-LOX acts differently in response to insect attacks with 1) distinct promoter activities 2) different signalling dynamics and 3) controlling different defences against herbivores.