Axial and Radial Oxylipin Transport

Article Article scientifique Article Axial and Radial Oxylipin Transport eng 2015 author Gasperini Debora author Chauvin Adeline 713430 author Acosta Ivan F. author Kurenda Andrzej author Stolz Stéphanie author Chételat Aurore author Wolfender Jean-Luc 135649 author Farmer Edward E 2244-2254 10.1104/pp.15.01104 26338953 0032-0889 Jasmonates are oxygenated lipids (oxylipins) that control defense gene expression in response to cell damage in plants. How mobile are these potent mediators within tissues? Exploiting a series of 13-lipoxygenase (13-lox) mutants in Arabidopsis (Arabidopsis thaliana) that displays impaired jasmonic acid (JA) synthesis in specific cell types and using JA-inducible reporters, we mapped the extent of the transport of endogenous jasmonates across the plant vegetative growth phase. In seedlings, we found that jasmonate (or JA precursors) could translocate axially from wounded shoots to unwounded roots in a LOX2-dependent manner. Grafting experiments with the wild type and JA-deficient mutants confirmed shoot-to-root oxylipin transport. Next, we used rosettes to investigate radial cell-to-cell transport of jasmonates. After finding that the LOX6 protein localized to xylem contact cells was not wound inducible, we used the lox234 triple mutant to genetically isolate LOX6 as the only JA precursor-producing LOX in the plant. When a leaf of this mutant was wounded, the JA reporter gene was expressed in distal leaves. Leaf sectioning showed that JA reporter expression extended from contact cells throughout the vascular bundle and into extravascular cells, revealing a radial movement of jasmonates. Our results add a crucial element to a growing picture of how the distal wound response is regulated in rosettes, showing that both axial (shoot-to-root) and radial (cell-to-cell) transport of oxylipins plays a major role in the wound response. The strategies developed herein provide unique tools with which to identify intercellular jasmonate transport routes. Arabidopsis/enzymology/genetics/physiology Arabidopsis Proteins/genetics/metabolism Biological Transport Cyclopentanes/metabolism Gene Expression Regulation, Plant Lipoxygenase/genetics/metabolism Lipoxygenases/genetics/metabolism Oxylipins/metabolism Plant Growth Regulators/metabolism Plant Leaves/enzymology/genetics/physiology Plant Roots/enzymology/genetics/physiology Plant Shoots/enzymology/genetics/physiology Seedlings/enzymology/genetics/physiology Stress, Physiological Xylem/enzymology/genetics/physiology Dewey 615 31 Section des sciences pharmaceutiques 17 false 882 Phytochimie et produits naturels bioactifs Plant Physiology 169 3 e7f7f1dc-d38a-4f54-a195-a03cfe58c306 Article (Published version) Restricted 26338953.pdf 1355026 application/pdf restrictedAccess Faculté des sciences / Section des sciences pharmaceutiques

Title	Published in	Year	Views	Downloads

Axial and Radial Oxylipin Transport	Plant Physiology	2015	396	0
A 13-LOX hierarchy for defence in Arabidopsis thaliana		2014	1,346	114

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Chauvin, Adeline