Optimization of neutral protease to collagenase activity ratio for islet of Langerhans isolation

Bucher, Pascal Alain Robert; Bosco, Domenico; Mathe, Z; Matthey-Doret, David; Andres, Axel; Kurfuerst, M; Rämsch-Günther, N; Buehler, Leo Hans; Morel, Philippe; Berney, Thierry

doi:10.1016/j.transproceed.2004.04.024

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Title		Optimization of neutral protease to collagenase activity ratio for islet of Langerhans isolation
Authors		Bucher, Pascal Alain Robert Bosco, Domenico Mathe, Z Matthey-Doret, David Andres, Axel Kurfuerst, M Rämsch-Günther, N Buehler, Leo Hans Morel, Philippe Berney, Thierry show all authors [1 - 10]
Published in		Transplantation Proceedings. 2004, vol. 36, no. 4, p. 1145-6
Abstract		The optimal neutral protease to collagenase activity ratio has not been determined for islet isolation. We evaluated a new highly purified collagenase that can be blended with predetermined amounts of neutral protease (NP).
Keywords		Animals — Candida albicans/isolation & purification — Cell Separation/methods — Fungi/isolation & purification — Gram-Negative Bacteria/isolation & purification — Gram-Positive Bacteria/isolation & purification — Islets of Langerhans/cytology/microbiology — Pancreas — Rats — Rats, Sprague-Dawley — Tissue and Organ Harvesting/methods
Identifiers		DOI: 10.1016/j.transproceed.2004.04.024 PMID: 15194399
Full text		Article (Published version) (50 Kb) - Limited access to UNIGE
Structures		Faculté de médecine / Section de médecine clinique / Département de chirurgie
Research groups		Chirurgie viscérale (104) Cultures cellulaires et transplantations (519) La transplantation d'îlots de Langerhans (623)
Citation (ISO format)		BUCHER, Pascal Alain Robert et al. Optimization of neutral protease to collagenase activity ratio for islet of Langerhans isolation. In: Transplantation Proceedings, 2004, vol. 36, n° 4, p. 1145-6. https://archive-ouverte.unige.ch/unige:41078