Optimization of neutral protease to collagenase activity ratio for islet of Langerhans isolation

Bucher, Pascal Alain Robert; Bosco, Domenico; Mathe, Z; Matthey-Doret, David; Andres, Axel; Kurfuerst, M; Rämsch-Günther, N; Buehler, Leo Hans; Morel, Philippe; Berney, Thierry

doi:10.1016/j.transproceed.2004.04.024

Scientific article

English

Optimization of neutral protease to collagenase activity ratio for islet of Langerhans isolation

ContributorsBucher, Pascal Alain Robert; Bosco, Domenico; Mathe, Z; Matthey-Doret, David; Andres, Axel; Kurfuerst, M; Rämsch-Günther, N; Buehler, Leo Hans; Morel, Philippe; Berney, Thierry

Published inTransplantation proceedings, vol. 36, no. 4, p. 1145-1146

Publication date2004

Abstract

The optimal neutral protease to collagenase activity ratio has not been determined for islet isolation. We evaluated a new highly purified collagenase that can be blended with predetermined amounts of neutral protease (NP).

Keywords

Animals
Candida albicans/isolation & purification
Cell Separation/methods
Fungi/isolation & purification
Gram-Negative Bacteria/isolation & purification
Gram-Positive Bacteria/isolation & purification
Islets of Langerhans/cytology/microbiology
Pancreas
Rats
Rats, Sprague-Dawley
Tissue and Organ Harvesting/methods

Affiliation entities

Faculté de médecine / Section de médecine clinique / Département de chirurgie

Research groups

Citation (ISO format)

BUCHER, Pascal Alain Robert et al. Optimization of neutral protease to collagenase activity ratio for islet of Langerhans isolation. In: Transplantation proceedings, 2004, vol. 36, n° 4, p. 1145–1146. doi: 10.1016/j.transproceed.2004.04.024

Article (Published version)

Identifiers

PID : unige:41078
DOI : 10.1016/j.transproceed.2004.04.024
PMID : 15194399

Journal ISSN0041-1345

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Optimization of neutral protease to collagenase activity ratio for islet of Langerhans isolation

The optimal neutral protease to collagenase activity ratio has not been determined for islet isolation. We evaluated a new highly purified collagenase that can be blended with predetermined amounts of neutral protease (NP).

Technical informations