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Scientific article
English

Optimization of neutral protease to collagenase activity ratio for islet of Langerhans isolation

Published inTransplantation proceedings, vol. 36, no. 4, p. 1145-1146
Publication date2004
Abstract

The optimal neutral protease to collagenase activity ratio has not been determined for islet isolation. We evaluated a new highly purified collagenase that can be blended with predetermined amounts of neutral protease (NP).

Keywords
  • Animals
  • Candida albicans/isolation & purification
  • Cell Separation/methods
  • Fungi/isolation & purification
  • Gram-Negative Bacteria/isolation & purification
  • Gram-Positive Bacteria/isolation & purification
  • Islets of Langerhans/cytology/microbiology
  • Pancreas
  • Rats
  • Rats, Sprague-Dawley
  • Tissue and Organ Harvesting/methods
Citation (ISO format)
BUCHER, Pascal Alain Robert et al. Optimization of neutral protease to collagenase activity ratio for islet of Langerhans isolation. In: Transplantation proceedings, 2004, vol. 36, n° 4, p. 1145–1146. doi: 10.1016/j.transproceed.2004.04.024
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Article (Published version)
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Identifiers
ISSN of the journal0041-1345
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