UNIGE document Scientific Article
previous document  unige:4081  next document
add to browser collection

The PAMPA technique as a HTS tool for partition coefficients determination in different solvent/water systems

Ottaviani, Giorgio
Escarala, Caroline
Published in European journal of pharmaceutical sciences. 2008, vol. 35, no. 1-2, p. 68-75
Abstract 1,2-dichloroethane (DCE) and o-nitrophenyl octyl ether (o-NPOE), were tested for their ability to form artificial membranes immobilized on polycarbonate (PC) and polyvinylidene fluoride (PVDF) supporting filters using the PAMPA (parallel artificial membrane permeability assays) technique. These detailed studies provided important information on the application domain of the artificial membranes investigated. According to the nature of the organic solvent and the composition of the filter, different permeation behaviours were noted. A double permeation pathway was observed for DCE-coated with PVDF filters since hydrophilic compounds permeated the membrane through aqueous pores created by the interaction of DCE and PVDF filters, while the more lipophilic compounds were trapped in the DCE present on filters. On the other hand, the permeation through PVDF and PC filters coated with o-NPOE did not follow the same mechanisms. An interesting application emerged from these mechanistic studies, namely the use of PC filters for a first high throughput assay designed to measure o-NPOE/water partition coefficients.
Keywords AlgorithmsChemistry, Pharmaceutical/methodsChemistry, Physical/methodsDrug Evaluation, Preclinical/methodsElectric ConductivityEthylene Dichlorides/chemistryFiltrationHydrogen-Ion ConcentrationMembranes, ArtificialPermeabilityPolycarboxylate Cement/chemistryPolyvinyls/chemistrySolubilitySolvents/chemistrySpectrophotometry, UltravioletWater/chemistry
PMID: 18620049
Full text
Article (Published version) (356 Kb) - document accessible for UNIGE members only Limited access to UNIGE
(ISO format)
OTTAVIANI, Giorgio et al. The PAMPA technique as a HTS tool for partition coefficients determination in different solvent/water systems. In: European journal of pharmaceutical sciences, 2008, vol. 35, n° 1-2, p. 68-75. doi: 10.1016/j.ejps.2008.06.006 https://archive-ouverte.unige.ch/unige:4081

540 hits



Deposited on : 2009-11-20

Export document
Format :
Citation style :