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Scientific article
English

UPLC-TOF-MS for plant metabolomics: a sequential approach for wound marker analysis in Arabidopsis thaliana

Published inJournal of chromatography. B, vol. 871, no. 2, p. 261-270
Publication date2008
Abstract

The model plant Arabidopsis thaliana was studied for the search of new metabolites involved in wound signalling. Diverse LC approaches were considered in terms of efficiency and analysis time and a 7-min gradient on a UPLC-TOF-MS system with a short column was chosen for metabolite fingerprinting. This screening step was designed to allow the comparison of a high number of samples over a wide range of time points after stress induction in positive and negative ionisation modes. Thanks to data treatment, clear discrimination was obtained, providing lists of potential stress-induced ions. In a second step, the fingerprinting conditions were transferred to longer column, providing a higher peak capacity able to demonstrate the presence of isomers among the highlighted compounds.

Keywords
  • Arabidopsis/metabolism
  • Chromatography, High Pressure Liquid/methods
  • Computational Biology/methods
  • Mass Spectrometry/methods
  • Plant Diseases
  • Plants/metabolism
  • Principal Component Analysis
Citation (ISO format)
GRATA, Elia et al. UPLC-TOF-MS for plant metabolomics: a sequential approach for wound marker analysis in Arabidopsis thaliana. In: Journal of chromatography. B, 2008, vol. 871, n° 2, p. 261–270. doi: 10.1016/j.jchromb.2008.04.021
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ISSN of the journal1570-0232
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Creation2009/11/06 10:25:00
First validation2009/11/06 10:25:00
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