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Scientific article
English

PNA-Encoded Synthesis (PES) of a 10 000-Member Hetero-Glycoconjugate Library and Microarray Analysis of Diverse Lectins

Published inChemBioChem, vol. 15, no. 14, p. 2058-2065
Publication date2014
Abstract

Identification of selective and synthetically tractable ligands to glycan-binding proteins is important in glycoscience. Carbohydrate arrays have had a tremendous impact on profiling glycan-binding proteins and as analytical tools. We report a highly miniaturized synthetic format to access nucleic-acid-encoded hetero-glycoconjugate libraries with an unprecedented diversity in the combinations of glycans, linkers, and capping groups. Novel information about plant and bacterial lectin specificity was obtained by microarray profiling, and we show that a ligand identified on the array can be converted to a high-affinity soluble ligand by straightforward chemistry.

Keywords
  • DNA display
  • Glycan array
  • Glycopeptides
  • Microarrays
  • Multivalency
  • PNA
Citation (ISO format)
NOVOA, Alexandre et al. PNA-Encoded Synthesis (PES) of a 10 000-Member Hetero-Glycoconjugate Library and Microarray Analysis of Diverse Lectins. In: ChemBioChem, 2014, vol. 15, n° 14, p. 2058–2065. doi: 10.1002/cbic.201402280
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Article (Published version)
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Identifiers
ISSN of the journal1439-4227
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