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Title

PNA-Encoded Synthesis (PES) of a 10 000-Member Hetero-Glycoconjugate Library and Microarray Analysis of Diverse Lectins
Authors
Novoa, Alexandre
Machida, Takuya
Barluenga Badiola, Sofia
Imberty, Anne
Winssinger, Nicolas
Published in ChemBioChem. 2014, vol. 15, no. 14, p. 2058-2065
Abstract Identification of selective and synthetically tractable ligands to glycan-binding proteins is important in glycoscience. Carbohydrate arrays have had a tremendous impact on profiling glycan-binding proteins and as analytical tools. We report a highly miniaturized synthetic format to access nucleic-acid-encoded hetero-glycoconjugate libraries with an unprecedented diversity in the combinations of glycans, linkers, and capping groups. Novel information about plant and bacterial lectin specificity was obtained by microarray profiling, and we show that a ligand identified on the array can be converted to a high-affinity soluble ligand by straightforward chemistry.
Keywords DNA display — Glycan array — Glycopeptides — Microarrays — Multivalency — PNA
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Structures
Faculté des sciences / Section de chimie et biochimie / Département de chimie organique
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(ISO format) 		NOVOA, Alexandre et al. PNA-Encoded Synthesis (PES) of a 10 000-Member Hetero-Glycoconjugate Library and Microarray Analysis of Diverse Lectins. In: ChemBioChem, 2014, vol. 15, n° 14, p. 2058-2065. doi: 10.1002/cbic.201402280 https://archive-ouverte.unige.ch/unige:40714

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Deposited on : 2014-10-06

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