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Scientific article
Open access
English

Expression, phosphorylation and function of the Rab-GTPase activating protein TBC1D1 in pancreatic beta-cells

Published inFEBS letters, vol. 588, no. 1, p. 15-20
Publication date2014
Abstract

The Rab-GTPase activating protein TBC1D1 is a paralog of AS160/TBC1D4. AS160/TBC1D4, a downstream effector of Akt, has been shown to play a central role in beta-cell function and survival. The two proteins have overlapping function in insulin signalling in muscle cells. However, the expression and the potential role of TBC1D1 in beta-cells remain unknown. Therefore, the aim of this study is to investigate whether TBC1D1 is expressed in beta-cells and whether it plays, as AS160/TBC1D4, a role in beta-cell function and survival. Using human and rat beta-cells, this study shows for the first time that TBC1D1 is expressed and phosphorylated in response to glucose in these cells. Knockdown of TBC1D1 in beta-cells resulted in increased basal and glucose-stimulated insulin release, decreased proliferation but no change in apoptosis.

Keywords
  • Animals
  • Apoptosis/genetics
  • Blotting, Western
  • Cell Proliferation
  • Cell Survival/genetics
  • Cells, Cultured
  • GTPase-Activating Proteins/genetics/metabolism
  • Gene Expression
  • Glucose/pharmacology
  • Humans
  • Insulin/secretion
  • Insulin-Secreting Cells/drug effects/metabolism
  • Male
  • Microscopy, Confocal
  • Phosphorylation/drug effects
  • Proteins
  • RNA Interference
  • Rats
  • Rats, Wistar
  • Reverse Transcriptase Polymerase Chain Reaction
Citation (ISO format)
RUTTI ROCH, Sabine et al. Expression, phosphorylation and function of the Rab-GTPase activating protein TBC1D1 in pancreatic beta-cells. In: FEBS letters, 2014, vol. 588, n° 1, p. 15–20. doi: 10.1016/j.febslet.2013.10.050
Main files (1)
Article (Accepted version)
accessLevelPublic
Identifiers
ISSN of the journal0014-5793
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343downloads

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