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Title

Expression, phosphorylation and function of the Rab-GTPase activating protein TBC1D1 in pancreatic beta-cells
Authors
Rutti Roch, Sabine
Arous, Caroline
Nica, Alexandra C
Kanzaki, Makoto
Halban, Philippe A.
Bouzakri, Karim
Published in FEBS Letters. 2014, vol. 588, no. 1, p. 15-20
Abstract The Rab-GTPase activating protein TBC1D1 is a paralog of AS160/TBC1D4. AS160/TBC1D4, a downstream effector of Akt, has been shown to play a central role in beta-cell function and survival. The two proteins have overlapping function in insulin signalling in muscle cells. However, the expression and the potential role of TBC1D1 in beta-cells remain unknown. Therefore, the aim of this study is to investigate whether TBC1D1 is expressed in beta-cells and whether it plays, as AS160/TBC1D4, a role in beta-cell function and survival. Using human and rat beta-cells, this study shows for the first time that TBC1D1 is expressed and phosphorylated in response to glucose in these cells. Knockdown of TBC1D1 in beta-cells resulted in increased basal and glucose-stimulated insulin release, decreased proliferation but no change in apoptosis.
Keywords Animals — Apoptosis/genetics — Blotting, Western — Cell Proliferation — Cell Survival/genetics — Cells, Cultured — GTPase-Activating Proteins/genetics/metabolism — Gene Expression — Glucose/pharmacology — Humans — Insulin/secretion — Insulin-Secreting Cells/drug effects/metabolism — Male — Microscopy, Confocal — Phosphorylation/drug effects — Proteins — RNA Interference — Rats — Rats, Wistar — Reverse Transcriptase Polymerase Chain Reaction
Identifiers
PMID: 24239544
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Article (Accepted version) (374 Kb) - public document Free access
Structures
Faculté de médecine / Section de médecine fondamentale / Département de médecine génétique et développement
Research group Fonction de l'îlot de Langerhans (324)
Citation
(ISO format) 		RUTTI ROCH, Sabine et al. Expression, phosphorylation and function of the Rab-GTPase activating protein TBC1D1 in pancreatic beta-cells. In: FEBS Letters, 2014, vol. 588, n° 1, p. 15-20. doi: 10.1016/j.febslet.2013.10.050 https://archive-ouverte.unige.ch/unige:40330

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Deposited on : 2014-09-18

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