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Expression, phosphorylation and function of the Rab-GTPase activating protein TBC1D1 in pancreatic beta-cells

Nica, Alexandra C
Kanzaki, Makoto
Published in FEBS letters. 2014, vol. 588, no. 1, p. 15-20
Abstract The Rab-GTPase activating protein TBC1D1 is a paralog of AS160/TBC1D4. AS160/TBC1D4, a downstream effector of Akt, has been shown to play a central role in beta-cell function and survival. The two proteins have overlapping function in insulin signalling in muscle cells. However, the expression and the potential role of TBC1D1 in beta-cells remain unknown. Therefore, the aim of this study is to investigate whether TBC1D1 is expressed in beta-cells and whether it plays, as AS160/TBC1D4, a role in beta-cell function and survival. Using human and rat beta-cells, this study shows for the first time that TBC1D1 is expressed and phosphorylated in response to glucose in these cells. Knockdown of TBC1D1 in beta-cells resulted in increased basal and glucose-stimulated insulin release, decreased proliferation but no change in apoptosis.
Keywords AnimalsApoptosis/geneticsBlotting, WesternCell ProliferationCell Survival/geneticsCells, CulturedGTPase-Activating Proteins/genetics/metabolismGene ExpressionGlucose/pharmacologyHumansInsulin/secretionInsulin-Secreting Cells/drug effects/metabolismMaleMicroscopy, ConfocalPhosphorylation/drug effectsProteinsRNA InterferenceRatsRats, WistarReverse Transcriptase Polymerase Chain Reaction
PMID: 24239544
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Research group Fonction de l'îlot de Langerhans (324)
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RUTTI ROCH, Sabine et al. Expression, phosphorylation and function of the Rab-GTPase activating protein TBC1D1 in pancreatic beta-cells. In: FEBS letters, 2014, vol. 588, n° 1, p. 15-20. doi: 10.1016/j.febslet.2013.10.050 https://archive-ouverte.unige.ch/unige:40330

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Deposited on : 2014-09-18

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