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Scientific article
English

Magnetic resonance imaging with hepatospecific contrast agents in cirrhotic rat livers

Published inInvestigative radiology, vol. 40, no. 4, p. 187-194
Publication date2005-04
Abstract

Objective: During biliary cirrhosis in rats, organic anion-transporting peptides (Oatps) and ATP-dependent multidrug resistance-associated protein 2 (Mrp2) that are likely to transport the contrast agent Gd-BOPTA through hepatocytes are down-regulated. However, the consequences of such down-regulation on the signal intensity (SI) enhancement are unknown. Consequently, the aim of our study was to measure the hepatic SI enhancement during Gd-BOPTA perfusion as well as the Oatp and Mrp2 expression in normal and cirrhotic livers.

Materials and methods: The hepatic SI enhancement during Gd-BOPTA perfusion was measured in livers isolated from normal rats and rats that had a bile duct ligation (BDL) 15, 30, and 60 days before the perfusion. Hepatic injury and transporter expression were measured in control and cirrhotic rats.

Results: BDL induced a severe hepatic injury that increased over time with a down-regulation of the transporter expression. The extracellular space (assessed by Gd-DTPA perfusion) increased with the severity of the disease. Gd-BOPTA-induced SI enhancement remained similar in BDL-15 and BDL-30 rats than in control rats but significantly decreased in severe cirrhosis (BDL-60 rats). In comparison, the Mn-DPDP-induced SI enhancement decreases proportionally to the severity of the disease.

Conclusion: During biliary cirrhosis, Gd-BOPTA-induced SI enhancement could not be related to the hepatic expression of transporters.

eng
Keywords
  • ATP-Binding Cassette Transporters/analysis
  • Albumins/analysis
  • Animals
  • Bile Ducts/physiology
  • Blotting, Western
  • Contrast Media
  • Keratins/analysis
  • Liver Cirrhosis, Experimental/diagnosis
  • Magnetic Resonance Imaging
  • Meglumine/analogs & derivatives/diagnostic use
  • Organic Anion Transporters/analysis
  • Organometallic Compounds/diagnostic use
  • Perfusion
  • Rats
  • Rats, Sprague-Dawley
Citation (ISO format)
PLANCHAMP MESSEILLER, Corinne et al. Magnetic resonance imaging with hepatospecific contrast agents in cirrhotic rat livers. In: Investigative radiology, 2005, vol. 40, n° 4, p. 187–194. doi: 10.1097/01.rli.0000154587.00638.77
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Article (Published version)
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Identifiers
ISSN of the journal0020-9996
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314downloads

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