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Assessment of pathogenic bacteria in water and sediment from a water reservoir under tropical conditions (Lake Ma Vallée), Kinshasa Democratic Republic of Congo

Published inEnvironmental monitoring and assessment, vol. 186, no. 10, p. 6821-6830
Publication date2014
Abstract

This study was conducted to assess potential human health risks presented by pathogenic bacteria in a protected multi-use lake-reservoir (Lake Ma Vallée) located in west of Kinshasa, Democratic Republic of Congo (DRC). Water and surface sediments from several points of the Lake were collected during summer. Microbial analysis was performed for Escherichia coli, Enterococcus (ENT), Pseudomonas species and heterotrophic plate counts. PCR amplification was performed for the confirmation of E. coli, ENT, Pseudomonas spp. and Pseudomonas aeruginosa isolated from samples. The results reveal low concentration of bacteria in water column of the lake, the bacterial quantification results observed in this study for the water column were below the recommended limits, according to WHO and the European Directive 2006/7/CE, for bathing water. However, high concentration of bacteria was observed in the sediment samples; the values of 2.65 × 103, 6.35 × 103, 3.27 × 103 and 3.60 × 108 CFU g−1 of dry sediment for E. coli, ENT, Pseudomonas spp. and heterotrophic plate counts, respectively. The results of this study indicate that sediments of the Lake Ma Vallée can constitute a reservoir of pathogenic microorganisms which can persist in the lake. Possible resuspension of faecal indicator bacteria and pathogens would affect water quality and may increase health risks to the population during recreational activities. Our results indicate that the microbial sediment analysis provides complementary and important information for assessing sanitary quality of surface water under tropical conditions.

Keywords
  • Lake Ma Vallée
  • Microbial contamination
  • Faecal indicator bacteria
  • Pseudomonas
  • Human health risk
  • Democratic Republic of Congo
Funding
  • Swiss National Science Foundation - Swiss National Science Foundation (grant no. 31003A_150163/1)
Citation (ISO format)
MWANAMOKI, Paola M. et al. Assessment of pathogenic bacteria in water and sediment from a water reservoir under tropical conditions (Lake Ma Vallée), Kinshasa Democratic Republic of Congo. In: Environmental monitoring and assessment, 2014, vol. 186, n° 10, p. 6821–6830. doi: 10.1007/s10661-014-3891-6
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ISSN of the journal0167-6369
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