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Involvement of the AP-1 adaptor complex in early steps of phagocytosis and macropinocytosis

Lefkir, Yaya
Malbouyres, Marilyne
Gotthardt, Daniel
Ozinsky, Adrian
Bruckert, Franz
Aderem, Alan A
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Published in Molecular biology of the cell. 2004, vol. 15, no. 2, p. 861-869
Abstract The best described function of the adaptor complex-1 (AP-1) is to participate in the budding of clathrin-coated vesicles from the trans-Golgi network and endosomes. Here, we show that AP-1 is also localized to phagocytic cups in murine macrophages as well as in Dictyostelium amoebae. AP-1 is recruited to phagosomal membranes at this early stage of phagosome formation and rapidly dissociates from maturing phagosomes. To establish the role of AP-1 in phagocytosis, we made used of Dictyostelium mutant cells (apm1(-) cells) disrupted for AP-1 medium chain. In this mutant, phagocytosis drops by 60%, indicating that AP-1 is necessary for efficient phagocytosis. Furthermore, phagocytosis in apm1(-) cells is more affected for large rather than small particles, and cells exhibiting incomplete engulfment are then often observed. This suggests that AP-1 could participate in the extension of the phagocytic cup. Interestingly, macropinocytosis, a process dedicated to fluid-phase endocytosis and related to phagocytosis, is also impaired in apm1(-) cells. In summary, our data suggest a new role of AP-1 at an early stage of phagosome and macropinosome formation.
Keywords AnimalsClathrin-Coated Vesicles/metabolismDictyostelium/metabolismEndosomes/metabolismGolgi Apparatus/metabolismMacrophages/metabolismMiceMicroscopyElectronScanningMutationPhagocytosis/physiologyPhagosomes/metabolismPinocytosis/physiologyTranscription Factor AP-1/metabolism
PMID: 14617812
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Research group Phagocytose (140)
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LEFKIR, Yaya et al. Involvement of the AP-1 adaptor complex in early steps of phagocytosis and macropinocytosis. In: Molecular biology of the cell, 2004, vol. 15, n° 2, p. 861-869. doi: 10.1091/mbc.E03-06-0365 https://archive-ouverte.unige.ch/unige:38877

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Deposited on : 2014-07-23

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