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Doctoral thesis
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Proteomic characterisation of the Mycobacterium marinum-containing compartment in Dictyostelium discoideum

Contributors

Gueho, Aurélie

Directors

Soldati, Thierry

Defense date

2013-10-10

Abstract

Dictyostelium discoideum is a professional phagocyte using phagocytosis to feed on bacteria. It is used as a surrogate macrophage to study phagocytosis mechanisms and as a host model organism to study intracellular bacterial infections. Mycobacterium marinum, as its close cousin M. tuberculosis, infects innate immune cells where it stops the maturation of its residing-phagosome to prevent its killing and to establish a compartment where it can replicate. In the following work, we have used the host-pathogen model system D. discoideum-M. marinum to better understand how M. marinum manipulates the maturation of its containing-phagosome. We have established a procedure to isolate mycobacteria-containing compartments. Then, using isobaric labelling and mass spectrometry, we studied quantitative proteomic modifications applied to the M. marinum-containing compartment during the first hours of infection. We also compared the quantitative proteomic composition of compartments containing the pathogenic strain M. marinum to compartments containing non-pathogenic, avirulent or attenuated mycobacteria strains.

Keywords

  • Dictyostelium
  • Mycobacteria
  • Phagosome maturation
  • Proteomic

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

GUEHO, Aurélie. Proteomic characterisation of the Mycobacterium marinum-containing compartment in Dictyostelium discoideum. Doctoral Thesis, 2013. doi: 10.13097/archive-ouverte/unige:37210

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02/06/2014 15:16:00

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02/06/2014 15:16:00

Update

14/03/2023 21:18:00

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