UNIGE document Scientific Article
previous document  unige:36910  next document
add to browser collection

The yeast SWISS-2DPAGE database

Published in Electrophoresis. 1996, vol. 17, no. 3, p. 556-65
Abstract The systematic sequencing of the yeast genome will soon be completed. A new challenge has been launched by the EUROFAN (European Functional Analysis) project whose goal is to elucidate the physiological and biochemical function of newly discovered open reading frames (ORF) from yeast. One of the approaches is to use protein-based technologies such as two-dimensional gel electrophoresis and protein identification in order to establish a yeast reference map. Modified protein patterns can be compared to the reference map which hopefully will help identify changes related, for example, to growth processes or developmental events. This paper describes the yeast SWISS-2DPAGE database in which charge separation was obtained using immobilized pH gradient (IPG). Proteins identified by gel comparison, amino acid composition analysis and/or microsequencing are recorded and described in an accessible uniform format. We have identified more than one hundred polypeptides, several of which were newly mapped. In addition, the yeast SWISS-2DPAGE database can be freely accessed through the World Wide Web (WWW) network on the ExPASy molecular biology server.
Keywords Databases, FactualElectrophoresis, Gel, Two-DimensionalFungal Proteins/chemistryMolecular Sequence DataPeptide MappingSaccharomyces cerevisiae/chemistry
PMID: 8740180
Full text
Article (Published version) (1.1 MB) - document accessible for UNIGE members only Limited access to UNIGE
Research groups Swiss-Prot Research Group
Calipho (80)
Chimie et protéomique clinique (270)
Groupe de Recherche Translationnelle en Biomarqueurs (635)
(ISO format)
SANCHEZ, Jean-Charles et al. The yeast SWISS-2DPAGE database. In: Electrophoresis, 1996, vol. 17, n° 3, p. 556-65. doi: 10.1002/elps.1150170326 https://archive-ouverte.unige.ch/unige:36910

500 hits

0 download


Deposited on : 2014-05-26

Export document
Format :
Citation style :