en
Scientific article
English

The yeast SWISS-2DPAGE database

Published inElectrophoresis, vol. 17, no. 3, p. 556-565
Publication date1996
Abstract

The systematic sequencing of the yeast genome will soon be completed. A new challenge has been launched by the EUROFAN (European Functional Analysis) project whose goal is to elucidate the physiological and biochemical function of newly discovered open reading frames (ORF) from yeast. One of the approaches is to use protein-based technologies such as two-dimensional gel electrophoresis and protein identification in order to establish a yeast reference map. Modified protein patterns can be compared to the reference map which hopefully will help identify changes related, for example, to growth processes or developmental events. This paper describes the yeast SWISS-2DPAGE database in which charge separation was obtained using immobilized pH gradient (IPG). Proteins identified by gel comparison, amino acid composition analysis and/or microsequencing are recorded and described in an accessible uniform format. We have identified more than one hundred polypeptides, several of which were newly mapped. In addition, the yeast SWISS-2DPAGE database can be freely accessed through the World Wide Web (WWW) network on the ExPASy molecular biology server.

Keywords
  • Databases, Factual
  • Electrophoresis, Gel, Two-Dimensional
  • Fungal Proteins/chemistry
  • Molecular Sequence Data
  • Peptide Mapping
  • Saccharomyces cerevisiae/chemistry
Citation (ISO format)
SANCHEZ, Jean-Charles et al. The yeast SWISS-2DPAGE database. In: Electrophoresis, 1996, vol. 17, n° 3, p. 556–565. doi: 10.1002/elps.1150170326
Main files (1)
Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal0173-0835
18views
0downloads

Technical informations

Creation05/16/2014 12:04:00 PM
First validation05/16/2014 12:04:00 PM
Update time03/30/2023 10:22:38 AM
Status update03/30/2023 10:22:37 AM
Last indexation01/16/2024 10:44:40 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack