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Title

The yeast SWISS-2DPAGE database
Authors
Sanchez, Jean-Charles
Golaz, Olivier Georges
Frutiger, Séverine
Schaller, Dominique
Appel, Ron David
Bairoch, Amos Marc
Hughes, Graham
Hochstrasser, Denis
Published in Electrophoresis. 1996, vol. 17, no. 3, p. 556-65
Abstract The systematic sequencing of the yeast genome will soon be completed. A new challenge has been launched by the EUROFAN (European Functional Analysis) project whose goal is to elucidate the physiological and biochemical function of newly discovered open reading frames (ORF) from yeast. One of the approaches is to use protein-based technologies such as two-dimensional gel electrophoresis and protein identification in order to establish a yeast reference map. Modified protein patterns can be compared to the reference map which hopefully will help identify changes related, for example, to growth processes or developmental events. This paper describes the yeast SWISS-2DPAGE database in which charge separation was obtained using immobilized pH gradient (IPG). Proteins identified by gel comparison, amino acid composition analysis and/or microsequencing are recorded and described in an accessible uniform format. We have identified more than one hundred polypeptides, several of which were newly mapped. In addition, the yeast SWISS-2DPAGE database can be freely accessed through the World Wide Web (WWW) network on the ExPASy molecular biology server.
Keywords Databases, Factual — Electrophoresis, Gel, Two-Dimensional — Fungal Proteins/chemistry — Molecular Sequence Data — Peptide Mapping — Saccharomyces cerevisiae/chemistry
Identifiers
PMID: 8740180
Full text
Article (Published version) (1.1 MB) - document accessible for UNIGE members only Limited access to UNIGE
Other version: http://onlinelibrary.wiley.com/doi/10.1002/elps.1150170326/abstract
Structures
Faculté de médecine / Section de médecine fondamentale / Département de biologie structurale et bioinformatique
Research groups Calipho (80)
Swiss-Prot Research Group
Chimie et protéomique clinique (270)
Groupe de Protéomique biomédicale (635)
Citation
(ISO format) 		SANCHEZ, Jean-Charles et al. The yeast SWISS-2DPAGE database. In: Electrophoresis, 1996, vol. 17, n° 3, p. 556-65. https://archive-ouverte.unige.ch/unige:36910

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