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Scientific article
English

Complementation of the DNA repair defect in xeroderma pigmentosum group G cells by a human cDNA related to yeast RAD2

Contributors

Scherly, Daniel; Nouspikel, Thierry; Corlet, Janine; Ucla, Catherine; Bairoch, Amos Marc; Clarkson, Stuart Gordon

Published in

Nature, vol. 363, no. 6425, p. 182-185

Publication date

1993

Abstract

Defects in human DNA repair proteins can give rise to the autosomal recessive disorders xeroderma pigmentosum (XP) and Cockayne's syndrome (CS), sometimes even together. Seven XP and three CS complementation groups have been identified that are thought to be due to mutations in genes from the nucleotide excision repair pathway. Here we isolate frog and human complementary DNAs that encode proteins resembling RAD2, a protein involved in this pathway in yeast. Alignment of these three polypeptides, together with two other RAD2 related proteins, reveals that their conserved sequences are largely confined to two regions. Expression of the human cDNA in vivo restores to normal the sensitivity to ultraviolet light and unscheduled DNA synthesis of lymphoblastoid cells from XP group G, but not CS group A. The XP-G correcting protein XPGC is generated from a messenger RNA of approximately 4 kilobases that is present in normal amounts in the XP-G cell line.

Keywords

  • Amino Acid Sequence
  • Animals
  • Cell Line
  • Cloning, Molecular
  • Cockayne Syndrome/genetics
  • DNA Repair/genetics/radiation effects
  • DNA-Binding Proteins
  • Endodeoxyribonucleases
  • Fungal Proteins/genetics
  • Genetic Complementation Test
  • Humans
  • Lupus Erythematosus, Systemic/genetics
  • Molecular Sequence Data
  • Saccharomyces cerevisiae/genetics
  • Saccharomyces cerevisiae Proteins
  • Sequence Alignment
  • Ultraviolet Rays
  • Xenopus laevis
  • Xeroderma Pigmentosum/genetics

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

SCHERLY, Daniel et al. Complementation of the DNA repair defect in xeroderma pigmentosum group G cells by a human cDNA related to yeast RAD2. In: Nature, 1993, vol. 363, n° 6425, p. 182–185. doi: 10.1038/363182a0

Main files (1)

Article (Published version)

Identifiers

Journal ISSN

0028-0836
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Technical informations

Creation

08/05/2014 11:37:00

First validation

08/05/2014 11:37:00

Update

14/03/2023 21:12:34

Status update

14/03/2023 21:12:33

Last indexation

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