UNIGE document Scientific Article
previous document  unige:36260  next document
add to browser collection
Title

cis-diol dehydrogenases encoded by the TOL pWW0 plasmid xylL gene and the Acinetobacter calcoaceticus chromosomal benD gene are members of the short-chain alcohol dehydrogenase superfamily

Authors
Neidle, Ellen L
Hartnett, Christopher
Ornston, L Nicholas
Rekik, Monique
Harayama, Shigeaki
Published in European Journal of Biochemistry. 1992, vol. 204, no. 1, p. 113-20
Abstract In the aerobic degradation of benzoate by bacteria, benzoate is first dihydroxylated by a ring-hydroxylating dioxygenase to form a cis-diol (1,2-dihydroxycyclohexa-3,4-diene carboxylate) which is subsequently transformed to a catechol by an NAD(+)-dependent cis-diol dehydrogenase. The structural gene for this dehydrogenase, encoded on TOL plasmid pWW0 of Pseudomonas putida (xylL) and that encoded on the chromosome of Acinetobacter calcoaceticus (benD), were sequenced. They encode polypeptides of about 28 kDa in size. These proteins are similar to each other, exhibiting 58% sequence identity. They are also similar to other proteins of at least 20 different functions, which are members of the short-chain alcohol dehydrogenase family. The alignment of these proteins suggest two amino acids, lysine and tyrosine, as catalytically important residues.
Keywords Acinetobacter calcoaceticus/enzymology/geneticsAlcohol Dehydrogenase/chemistry/geneticsAmino Acid SequenceBase SequenceBenzoates/metabolismBenzoic AcidCatechols/metabolismCodonGenes, BacterialMolecular Sequence DataOxidoreductases/chemistry/geneticsOxidoreductases Acting on CH-CH Group DonorsPlasmidsPseudomonas putida/enzymology/geneticsSequence Homology, Nucleic Acid
Identifiers
PMID: 1740120
Full text
Article (Published version) (751 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Research group Calipho (80)
Citation
(ISO format)
NEIDLE, Ellen L et al. cis-diol dehydrogenases encoded by the TOL pWW0 plasmid xylL gene and the Acinetobacter calcoaceticus chromosomal benD gene are members of the short-chain alcohol dehydrogenase superfamily. In: European Journal of Biochemistry, 1992, vol. 204, n° 1, p. 113-20. https://archive-ouverte.unige.ch/unige:36260

172 hits

0 download

Update

Deposited on : 2014-05-05

Export document
Format :
Citation style :