Article (Published version) (765 Kb) - 
Limited access to UNIGE
Highlights
Home
Titles listAmino acid sequence determination by gas chromatography—mass spectrometry of permethylated peptides
Titles listAmino acid sequence determination by gas chromatography—mass spectrometry of permethylated peptides
Scientific Article
unige:36226 
 |
Amino acid sequence determination by gas chromatography—mass spectrometry of permethylated peptides |
|
| Authors | ||
| Published in | Journal of chromatography. 1983, vol. 268, p. 197-206 | |
| Abstract | The application of capillary columns to the determination of the amino acid sequence of proteins and polypeptides by gas chromatography-mass spectrometry (GC-MS) of partial hydrolysates is described and discussed for the case of the Napetrifluoroacetyl-N,O-permethyl derivatives. Retention indices are determined with the aid of a computer program. The mass spectra of methionine enkephalin obtained by GC-MS on a packed and on a capillary column are presented and a retention index calculated. Amounts of derivative corresponding to sub-nanomole amounts of peptides are sufficient to provide full amino acid sequence information. To assist in the assignment of retention indices above 4400, a mixture of n-alkanes from a commercial source was characterized by GC-MS up to n-C54H110 and was found to contain material up to an expected C72H146. | |
| Identifiers | ||
| Full text | ||
| Structures | ||
| Research group | Calipho (80) | |
| Citation (ISO format) | ROSE, Keith, BAIROCH, Amos Marc, OFFORD, Robin Ewart. Amino acid sequence determination by gas chromatography—mass spectrometry of permethylated peptides. In: Journal of chromatography, 1983, vol. 268, p. 197-206. doi: 10.1016/S0021-9673(01)95406-8 https://archive-ouverte.unige.ch/unige:36226 |
562 hits 
0 download 
Contact an author
Update