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Amino acid sequence determination by gas chromatography—mass spectrometry of permethylated peptides |
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Published in | Journal of chromatography. 1983, vol. 268, p. 197-206 | |
Abstract | The application of capillary columns to the determination of the amino acid sequence of proteins and polypeptides by gas chromatography-mass spectrometry (GC-MS) of partial hydrolysates is described and discussed for the case of the Napetrifluoroacetyl-N,O-permethyl derivatives. Retention indices are determined with the aid of a computer program. The mass spectra of methionine enkephalin obtained by GC-MS on a packed and on a capillary column are presented and a retention index calculated. Amounts of derivative corresponding to sub-nanomole amounts of peptides are sufficient to provide full amino acid sequence information. To assist in the assignment of retention indices above 4400, a mixture of n-alkanes from a commercial source was characterized by GC-MS up to n-C54H110 and was found to contain material up to an expected C72H146. | |
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Research group | Calipho (80) | |
Citation (ISO format) | ROSE, Keith, BAIROCH, Amos Marc, OFFORD, Robin Ewart. Amino acid sequence determination by gas chromatography—mass spectrometry of permethylated peptides. In: Journal of chromatography, 1983, vol. 268, p. 197-206. doi: 10.1016/S0021-9673(01)95406-8 https://archive-ouverte.unige.ch/unige:36226 |