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Amino acid sequence determination by gas chromatography—mass spectrometry of permethylated peptides

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Published in Journal of Chromatography. A. 1983, vol. 268, p. 197-206
Abstract The application of capillary columns to the determination of the amino acid sequence of proteins and polypeptides by gas chromatography-mass spectrometry (GC-MS) of partial hydrolysates is described and discussed for the case of the Napetrifluoroacetyl-N,O-permethyl derivatives. Retention indices are determined with the aid of a computer program. The mass spectra of methionine enkephalin obtained by GC-MS on a packed and on a capillary column are presented and a retention index calculated. Amounts of derivative corresponding to sub-nanomole amounts of peptides are sufficient to provide full amino acid sequence information. To assist in the assignment of retention indices above 4400, a mixture of n-alkanes from a commercial source was characterized by GC-MS up to n-C54H110 and was found to contain material up to an expected C72H146.
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Research group Calipho (80)
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ROSE, Keith, BAIROCH, Amos Marc, OFFORD, Robin Ewart. Amino acid sequence determination by gas chromatography—mass spectrometry of permethylated peptides. In: Journal of Chromatography. A, 1983, vol. 268, p. 197-206. https://archive-ouverte.unige.ch/unige:36226

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Deposited on : 2014-04-30

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