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Title

Amino acid sequence determination by gas chromatography—mass spectrometry of permethylated peptides
Authors
Rose, Keith
Bairoch, Amos Marc
Offord, Robin Ewart
Published in Journal of Chromatography. A. 1983, vol. 268, p. 197-206
Abstract The application of capillary columns to the determination of the amino acid sequence of proteins and polypeptides by gas chromatography-mass spectrometry (GC-MS) of partial hydrolysates is described and discussed for the case of the Napetrifluoroacetyl-N,O-permethyl derivatives. Retention indices are determined with the aid of a computer program. The mass spectra of methionine enkephalin obtained by GC-MS on a packed and on a capillary column are presented and a retention index calculated. Amounts of derivative corresponding to sub-nanomole amounts of peptides are sufficient to provide full amino acid sequence information. To assist in the assignment of retention indices above 4400, a mixture of n-alkanes from a commercial source was characterized by GC-MS up to n-C54H110 and was found to contain material up to an expected C72H146.
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Article (Published version) (765 Kb) - document accessible for UNIGE members only Limited access to UNIGE
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Faculté de médecine / Section de médecine fondamentale / Département de biologie structurale et bioinformatique
Research group Calipho (80)
Citation
(ISO format) 		ROSE, Keith, BAIROCH, Amos Marc, OFFORD, Robin Ewart. Amino acid sequence determination by gas chromatography—mass spectrometry of permethylated peptides. In: Journal of Chromatography. A, 1983, vol. 268, p. 197-206. doi: 10.1016/S0021-9673(01)95406-8 https://archive-ouverte.unige.ch/unige:36226

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Deposited on : 2014-04-30

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