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Doctoral thesis
English

New Inisgths on Lipid Homeostasis: Systematic Lipidomics of a Yeast Knockout Collection of Genes of Protein Kinases and Phosphatases

Contributors

Xavier Da Silveira Dos Sa, Aline

Directors

Riezman, Howard

Defense date

2014-03-03

Abstract

The regulatory pathways required to maintain eukaryotic lipid homeostasis are still largely unknown, which limits the possibilities of hypothesis driven research. Here, we performed a mass spectrometry-based lipidomic screening of yeast strains knocked out for genes of protein kinases and phosphatases, in search for new regulators of lipid homeostasis. We have uncovered novel regulations of lipid homeostasis, revealing a control of acyl chain length by nutrient sensing pathways, a control of complex sphingolipids by a phosphate sensing pathway and new players in the control of sphingolipid metabolism. We showed that systematic lipidomics is a powerful tool to gain novel insights into lipid homeostasis. Due to close interaction between lipid metabolism and other cellular processes it is clear that many of these new lipidomic data from kinases and phosphatase mutants will facilitate the understanding of signaling networks.

Keywords

  • Lipidomics
  • Yeast
  • Kinases
  • Phosphatases
  • Sphingolipids
  • Glycerophospholipids
  • Sterols
  • MRM-MS
  • GC-MS

UNIGE affiliation entities

UNIGE research groups

Funding

  • Autre - LipidX

Citation (ISO format)

XAVIER DA SILVEIRA DOS SA, Aline. New Inisgths on Lipid Homeostasis: Systematic Lipidomics of a Yeast Knockout Collection of Genes of Protein Kinases and Phosphatases. Doctoral Thesis, 2014. doi: 10.13097/archive-ouverte/unige:35283

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Thesis

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Technical informations

Creation

31/03/2014 17:06:00

First validation

31/03/2014 17:06:00

Update

14/03/2023 21:05:04

Status update

14/03/2023 21:05:03

Last indexation

13/05/2025 16:33:15
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