New Inisgths on Lipid Homeostasis: Systematic Lipidomics of a Yeast Knockout Collection of Genes of Protein Kinases and Phosphatases

Xavier Da Silveira Dos Sa, Aline

doi:10.13097/archive-ouverte/unige:35283

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Title		New Inisgths on Lipid Homeostasis: Systematic Lipidomics of a Yeast Knockout Collection of Genes of Protein Kinases and Phosphatases
Author		Xavier Da Silveira Dos Sa, Aline
Director		Riezman, Howard
Defense		Thèse de doctorat : Univ. Genève, 2014 - Sc. 4650 - 2014/03/03
Abstract		The regulatory pathways required to maintain eukaryotic lipid homeostasis are still largely unknown, which limits the possibilities of hypothesis driven research. Here, we performed a mass spectrometry-based lipidomic screening of yeast strains knocked out for genes of protein kinases and phosphatases, in search for new regulators of lipid homeostasis. We have uncovered novel regulations of lipid homeostasis, revealing a control of acyl chain length by nutrient sensing pathways, a control of complex sphingolipids by a phosphate sensing pathway and new players in the control of sphingolipid metabolism. We showed that systematic lipidomics is a powerful tool to gain novel insights into lipid homeostasis. Due to close interaction between lipid metabolism and other cellular processes it is clear that many of these new lipidomic data from kinases and phosphatase mutants will facilitate the understanding of signaling networks.
Keywords		Lipidomics — Yeast — Kinases — Phosphatases — Sphingolipids — Glycerophospholipids — Sterols — MRM-MS — GC-MS
Identifiers		DOI: 10.13097/archive-ouverte/unige:35283 URN: urn:nbn:ch:unige-352834
Full text		Thesis (18.4 MB) - Limited access to UNIGE
Structures		Faculté des sciences / Section de chimie et biochimie / Département de biochimie
Research group		Groupe Riezman
Project		Autre: LipidX
Citation (ISO format)		XAVIER DA SILVEIRA DOS SA, Aline. New Inisgths on Lipid Homeostasis: Systematic Lipidomics of a Yeast Knockout Collection of Genes of Protein Kinases and Phosphatases. Université de Genève. Thèse, 2014. doi: 10.13097/archive-ouverte/unige:35283 https://archive-ouverte.unige.ch/unige:35283