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Title

The Rab3-interacting molecule RIM is expressed in pancreatic beta-cells and is implicated in insulin exocytosis

Authors
Regazzi, R
Published in FEBS Letters. 2000, vol. 474, no. 1, p. 66-70
Abstract The putative Rab3 effector RIM (Rab3-interacting molecule) was detected by Northern blotting, RT-PCR and Western blotting in native pancreatic beta-cells as well as in the derived cell lines INS-1E and HIT-T15. RIM was localized on the plasma membrane of INS-1E cells and beta-cells. An involvement of RIM in insulin exocytosis was indicated by transfection experiments of INS-1E cells with the Rab3 binding domain of RIM. This domain enhanced glucose-stimulated secretion in intact cells and Ca(2+)-stimulated exocytosis in permeabilized cells. Co-expression of Rab3A reversed the effect of RIM on exocytosis. These results suggest an implication of RIM in the control of insulin secretion.
Keywords AnimalsCell LineCell Membrane/chemistryCricetinaeExocytosisGTP-Binding ProteinsGene ExpressionHumansInsulin/secretionInsulinomaIslets of Langerhans/metabolism/ultrastructureMiceNerve Tissue Proteins/analysis/genetics/physiologyPancreatic NeoplasmsRatsReverse Transcriptase Polymerase Chain ReactionTumor Cells, CulturedRab3 GTP-Binding Proteins/metabolism
Identifiers
PMID: 10828453
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IEZZI-BAKHTIARI, Mariella, REGAZZI, R, WOLLHEIM, Claes. The Rab3-interacting molecule RIM is expressed in pancreatic beta-cells and is implicated in insulin exocytosis. In: FEBS Letters, 2000, vol. 474, n° 1, p. 66-70. https://archive-ouverte.unige.ch/unige:35093

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Deposited on : 2014-03-28

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