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Scientific article
Open access
English

The Rab3-interacting molecule RIM is expressed in pancreatic beta-cells and is implicated in insulin exocytosis

Published inFEBS letters, vol. 474, no. 1, p. 66-70
Publication date2000
Abstract

The putative Rab3 effector RIM (Rab3-interacting molecule) was detected by Northern blotting, RT-PCR and Western blotting in native pancreatic beta-cells as well as in the derived cell lines INS-1E and HIT-T15. RIM was localized on the plasma membrane of INS-1E cells and beta-cells. An involvement of RIM in insulin exocytosis was indicated by transfection experiments of INS-1E cells with the Rab3 binding domain of RIM. This domain enhanced glucose-stimulated secretion in intact cells and Ca(2+)-stimulated exocytosis in permeabilized cells. Co-expression of Rab3A reversed the effect of RIM on exocytosis. These results suggest an implication of RIM in the control of insulin secretion.

Keywords
  • Animals
  • Cell Line
  • Cell Membrane/chemistry
  • Cricetinae
  • Exocytosis
  • GTP-Binding Proteins
  • Gene Expression
  • Humans
  • Insulin/secretion
  • Insulinoma
  • Islets of Langerhans/metabolism/ultrastructure
  • Mice
  • Nerve Tissue Proteins/analysis/genetics/physiology
  • Pancreatic Neoplasms
  • Rats
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tumor Cells, Cultured
  • Rab3 GTP-Binding Proteins/metabolism
Citation (ISO format)
IEZZI-BAKHTIARI, Mariella, REGAZZI, Romano, WOLLHEIM, Claes. The Rab3-interacting molecule RIM is expressed in pancreatic beta-cells and is implicated in insulin exocytosis. In: FEBS letters, 2000, vol. 474, n° 1, p. 66–70. doi: 10.1016/s0014-5793(00)01572-6
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Article (Published version)
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Identifiers
ISSN of the journal0014-5793
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323downloads

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