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Scientific article
English

Characterization of the platelet granule proteome: Evidence of the presence of MHC1 in alpha-granules

Published inJournal of proteomics, vol. 101C, p. 130-140
Publication date2014
Abstract

In the present study, we performed an extensive qualitative characterization of the platelet granule proteome using subcellular fractionation followed by mass spectrometry analysis and functional annotation. Eight-hundred-and-twenty-seven proteins were identified, most of them being associated to granules and to the granule's secretory machinery. Functional pathway analysis revealed 30 pathways, including the major histocompatibility complex class 1 (MHC I) presenting antigen pathway. This pathway was of particular interest for its potential interrelation between platelets and the immune system. Key proteins belonging to this metabolic route such as β-2-microglobulin, 26S protease regulatory subunit 10B from the proteasome and proteins 1 and 2 of the transporter associated with antigen processing were shown to co-localize with von Willebrand factor in resting platelets and to be located on the plasma membrane when platelets were activated. Key proteins of the MHC1 antigen-presenting pathway are located in platelet alpha-granules. These results suggest a possible functional role of platelet granules in platelet-related immune modulation.

Citation (ISO format)
ZUFFEREY BAKOS, Anne et al. Characterization of the platelet granule proteome: Evidence of the presence of MHC1 in alpha-granules. In: Journal of proteomics, 2014, vol. 101C, p. 130–140. doi: 10.1016/j.jprot.2014.02.008
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ISSN of the journal1874-3919
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