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Title

Adenovirus-mediated silencing of synaptotagmin 9 inhibits Ca2+-dependent insulin secretion in islets

Authors
Eliasson, Lena
Fukuda, Mitsunori
Published in FEBS Letters. 2005, vol. 579, no. 23, p. 5241-6
Abstract Synaptotagmins (Syts) are involved in Ca(2+)-dependent insulin release. However, which Syt isoform is functional in primary beta-cells remains unknown. We demonstrate by electron microscopy of pancreatic islets, the association of Syt 9 with insulin granules. Silencing of Syt 9 by RNA interference adenovirus in islet cells had no effect on the expression of Syt 5, Syt 7 and Syt 3 isoforms. The latter was localized at the plasma membrane of pancreatic polypeptide cells. Insulin release in response to glucose or tolbutamide was strongly inhibited in Syt 9 deficient islets, whereas exocytosis potentiated by raising cAMP levels, was unaltered. Thus, Syt 9 may act as Ca(2+) sensor for beta-cell secretion.
Keywords Adenoviridae/genetics/metabolismAnimalsCalcium/metabolismCalcium-Binding Proteins/genetics/metabolismCell LineInsulin/metabolismIslets of Langerhans/metabolism/ultrastructureMembrane Glycoproteins/metabolismMiceNerve Tissue Proteins/metabolismProtein Isoforms/metabolismRNA InterferenceRatsSynaptotagmins
Identifiers
PMID: 16165130
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IEZZI-BAKHTIARI, Mariella et al. Adenovirus-mediated silencing of synaptotagmin 9 inhibits Ca2+-dependent insulin secretion in islets. In: FEBS Letters, 2005, vol. 579, n° 23, p. 5241-6. https://archive-ouverte.unige.ch/unige:34531

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Deposited on : 2014-02-28

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