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Scientific article
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English

Adenovirus-mediated silencing of synaptotagmin 9 inhibits Ca2+-dependent insulin secretion in islets

Published inFEBS letters, vol. 579, no. 23, p. 5241-5246
Publication date2005
Abstract

Synaptotagmins (Syts) are involved in Ca(2+)-dependent insulin release. However, which Syt isoform is functional in primary beta-cells remains unknown. We demonstrate by electron microscopy of pancreatic islets, the association of Syt 9 with insulin granules. Silencing of Syt 9 by RNA interference adenovirus in islet cells had no effect on the expression of Syt 5, Syt 7 and Syt 3 isoforms. The latter was localized at the plasma membrane of pancreatic polypeptide cells. Insulin release in response to glucose or tolbutamide was strongly inhibited in Syt 9 deficient islets, whereas exocytosis potentiated by raising cAMP levels, was unaltered. Thus, Syt 9 may act as Ca(2+) sensor for beta-cell secretion.

Keywords
  • Adenoviridae/genetics/metabolism
  • Animals
  • Calcium/metabolism
  • Calcium-Binding Proteins/genetics/metabolism
  • Cell Line
  • Insulin/metabolism
  • Islets of Langerhans/metabolism/ultrastructure
  • Membrane Glycoproteins/metabolism
  • Mice
  • Nerve Tissue Proteins/metabolism
  • Protein Isoforms/metabolism
  • RNA Interference
  • Rats
  • Synaptotagmins
Citation (ISO format)
IEZZI-BAKHTIARI, Mariella et al. Adenovirus-mediated silencing of synaptotagmin 9 inhibits Ca2+-dependent insulin secretion in islets. In: FEBS letters, 2005, vol. 579, n° 23, p. 5241–5246. doi: 10.1016/j.febslet.2005.08.047
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Article (Published version)
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ISSN of the journal0014-5793
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