

![]() |
Development of Mice with Brain-Specific Deletion of Floxed Glud1 (Glutamate Dehydrogenase 1) Using Cre Recombinase Driven by the Nestin Promoter |
|
Authors | ||
Published in | Neurochemical research. 2013, vol. 2, no. 5, p. 284-90 | |
Abstract | In the brain, Glud1-encoded glutamate dehydrogenase plays a major role in the recycling of the neurotransmitter glutamate. We recently reported a new model of brain-specific Glud1 null mice (Cns-Glud1 (-/-)) lacking glutamate dehydrogenase in the central nervous system. Cns-Glud1 (-/-) mice exhibit reduced astrocytic glutamate breakdown and redirection of glutamate pathways without altering synaptic transmission. Cns-Glud1 (-/-) mice were generated using LoxP and Nestin-Cre technology. Nestin-Cre mice are widely used to investigate gene deletion in the central nervous system. However, the Nes-Cre transgene itself was reported to induce a phenotype related to body weight gain. Here, we review the potential side-effects of Nes-Cre and analysed Cns-Glud1 (-/-) body weight gain. Overall, Nestin-Cre mice may exhibit transient and moderate growth retardation during the few weeks immediately following weaning. Pending appropriate controls and homogenization of the genetic background, Nestin-Cre technology is a valuable tool enabling disruption of genes of interest in the central nervous system. | |
Keywords | Axons/chemistry/physiology — Brain/anatomy & histology/physiology — Brain Chemistry/physiology — Brain Mapping/instrumentation/methods — Humans — Male — Middle Aged — Nerve Fibers, Myelinated/chemistry/physiology — Neural Pathways/chemistry/physiology — Pattern Recognition, Automated/methods | |
Identifiers | PMID: 23595828 | |
Full text | ||
Structures | ||
Research group | Mitochondries et métabolisme énergétique (671) | |
Citation (ISO format) | KARACA EMRE, Melis, MAECHLER, Pierre. Development of Mice with Brain-Specific Deletion of Floxed Glud1 (Glutamate Dehydrogenase 1) Using Cre Recombinase Driven by the Nestin Promoter. In: Neurochemical research, 2013, vol. 2, n° 5, p. 284-90. doi: 10.1007/s11064-013-1041-0 https://archive-ouverte.unige.ch/unige:33351 |