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Development of Mice with Brain-Specific Deletion of Floxed Glud1 (Glutamate Dehydrogenase 1) Using Cre Recombinase Driven by the Nestin Promoter

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Published in Neurochemical research. 2013, vol. 2, no. 5, p. 284-90
Abstract In the brain, Glud1-encoded glutamate dehydrogenase plays a major role in the recycling of the neurotransmitter glutamate. We recently reported a new model of brain-specific Glud1 null mice (Cns-Glud1 (-/-)) lacking glutamate dehydrogenase in the central nervous system. Cns-Glud1 (-/-) mice exhibit reduced astrocytic glutamate breakdown and redirection of glutamate pathways without altering synaptic transmission. Cns-Glud1 (-/-) mice were generated using LoxP and Nestin-Cre technology. Nestin-Cre mice are widely used to investigate gene deletion in the central nervous system. However, the Nes-Cre transgene itself was reported to induce a phenotype related to body weight gain. Here, we review the potential side-effects of Nes-Cre and analysed Cns-Glud1 (-/-) body weight gain. Overall, Nestin-Cre mice may exhibit transient and moderate growth retardation during the few weeks immediately following weaning. Pending appropriate controls and homogenization of the genetic background, Nestin-Cre technology is a valuable tool enabling disruption of genes of interest in the central nervous system.
Keywords Axons/chemistry/physiologyBrain/anatomy & histology/physiologyBrain Chemistry/physiologyBrain Mapping/instrumentation/methodsHumansMaleMiddle AgedNerve Fibers, Myelinated/chemistry/physiologyNeural Pathways/chemistry/physiologyPattern Recognition, Automated/methods
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PMID: 23595828
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Article (Published version) (322 Kb) - document accessible for UNIGE members only Limited access to UNIGE
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Research group Mitochondries et métabolisme énergétique (671)
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KARACA EMRE, Melis, MAECHLER, Pierre. Development of Mice with Brain-Specific Deletion of Floxed Glud1 (Glutamate Dehydrogenase 1) Using Cre Recombinase Driven by the Nestin Promoter. In: Neurochemical research, 2013, vol. 2, n° 5, p. 284-90. doi: 10.1007/s11064-013-1041-0 https://archive-ouverte.unige.ch/unige:33351

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Deposited on : 2014-01-16

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