en
Scientific article
English

Global analytical strategy to measure drug–plasma protein interactions: from high-throughput to in-depth analysis

Published inDrug discovery today, vol. 18, no. 21-22, p. 1030-1034
Publication date2013
Abstract

The selection of drug candidates with improved pharmacokinetics is essential to reduce the attrition rates during drug development and represents one of the big challenges faced by the pharmaceutical industry. Plasma protein binding (PPB) is an important parameter with significant implications for in vivo drug performance. Today, the most widely used techniques for PPB measurement in the pharmaceutical community are equilibrium dialysis (ED) and ultrafiltration (UF). However, these techniques have some limitations. Thus, we emphasize an alternative strategy, based on a global, new and easy-to-follow methodology, to screen and perform determination of PPB, using orthogonal techniques (i.e. liquid chromatography (LC), capillary electrophoresis (CE), surface plasmon resonance (SPR) based biosensor). We anticipate that the increased knowledge gained through this strategy will lead to improved drug candidates.

Citation (ISO format)
VUIGNIER, Karine et al. Global analytical strategy to measure drug–plasma protein interactions: from high-throughput to in-depth analysis. In: Drug discovery today, 2013, vol. 18, n° 21-22, p. 1030–1034. doi: 10.1016/j.drudis.2013.04.006
Main files (1)
Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal1359-6446
656views
0downloads

Technical informations

Creation12/23/2013 9:41:00 AM
First validation12/23/2013 9:41:00 AM
Update time03/14/2023 8:47:37 PM
Status update03/14/2023 8:47:36 PM
Last indexation01/16/2024 8:43:33 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack