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Title

Rapid identification of Mg-chelated chlorins by on-line high performance liquid chromatography/ atmospheric pressure chemical ionization mass spectrometry

Authors
Riffé-Chalard, Catherine
Published in Rapid Communications in Mass Spectrometry. 2000, vol. 14, no. 7, p. 590-594
Abstract On-line high performance liquid chromatography/atmospheric pressure chemical ionization mass spectrometry (HPLC/APCI-MS) has been applied to the identification of a number of tetrapyrrolic pigments as well as several magnesium-free analogs. Mass spectra, acquired both in positive (+) and negative (-) ionization mode, allow not only the determination of the structural features of the pigments, but also a very easy differentiation of the Mg-chelated pigments from the free bases. In the positive ionization mode, all pigments show mainly a [M + H]+ ion and a [M + H - C20H38]+ fragment ion corresponding to the loss of the phytyl chain via a hydrogen rearrangement. In the negative ionization mode, on the other hand, although all pigments give an abundant molecular anion [M]-·, only the Mg-chelated chlorin spectra show a prominent fragment [M - C20H39]- produced by a formal loss of the phytyl radical.
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Research group Groupe Gülaçar
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VERZEGNASSI, Ludovica, RIFFÉ-CHALARD, Catherine, GULACAR, Fazil. Rapid identification of Mg-chelated chlorins by on-line high performance liquid chromatography/ atmospheric pressure chemical ionization mass spectrometry. In: Rapid Communications in Mass Spectrometry, 2000, vol. 14, n° 7, p. 590-594. https://archive-ouverte.unige.ch/unige:3204

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Deposited on : 2009-09-21

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