A comparison of frailty of primary neurons, embryonic, and aging mouse cortical layers

Fugistier, Patrick; Vallet, Philippe G; Leuba, Geneviève; Piotton, Françoise; Marin, Pascale; Bouras, Constantin; Savioz, Armand

doi:10.1016/j.neurobiolaging.2013.08.005

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Title		A comparison of frailty of primary neurons, embryonic, and aging mouse cortical layers
Authors		Fugistier, Patrick Vallet, Philippe G Leuba, Geneviève Piotton, Françoise Marin, Pascale Bouras, Constantin Savioz, Armand
Published in		Neurobiology of Aging. 2014, vol. 35, no. 2, p. 322-330
Abstract		Superficial layers I to III of the human cerebral cortex are more vulnerable toward Aβ peptides than deep layers V to VI in aging. Three models of layers were used to investigate this pattern of frailty. First, primary neurons from E14 and E17 embryonic murine cortices, corresponding respectively to future deep and superficial layers, were treated either with Aβ1-42, okadaic acid, or kainic acid. Second, whole E14 and E17 embryonic cortices, and third, in vitro separated deep and superficial layers of young and old C57BL/6J mice, were treated identically. We observed that E14 and E17 neurons in culture were prone to death after the Aβ and particularly the kainic acid treatment. This was also the case for the superficial layers of the aged cortex, but not for the embryonic, the young cortex, and the deep layers of the aged cortex. Thus, the aged superficial layers appeared to be preferentially vulnerable against Aβ and kainic acid. This pattern of vulnerability corresponds to enhanced accumulation of senile plaques in the superficial cortical layers with aging and Alzheimer's disease.
Keywords		Abeta amyloid — Aging — Alzheimer’s disease — Cell viability — Cell death — Cerebral cortex — Cortical layers — Cortical vulnerability — Cux2 — Kainic acid — Okadaic acid — PSD-95
Identifiers		DOI: 10.1016/j.neurobiolaging.2013.08.005 PMID: 24011540
Full text		Article (Published version) (1.7 MB) - Limited access to UNIGE
Structures		Faculté de médecine / Section de médecine clinique / Département de psychiatrie
Citation (ISO format)		FUGISTIER, Patrick et al. A comparison of frailty of primary neurons, embryonic, and aging mouse cortical layers. In: Neurobiology of Aging, 2014, vol. 35, n° 2, p. 322-330. doi: 10.1016/j.neurobiolaging.2013.08.005 https://archive-ouverte.unige.ch/unige:31671