A comparison of frailty of primary neurons, embryonic, and aging mouse cortical layers

Article Article scientifique Article A comparison of frailty of primary neurons, embryonic, and aging mouse cortical layers eng 2014 author Fugistier Patrick 591664 author Vallet Philippe G author Leuba Geneviève author Piotton Françoise 22090 author Marin Pascale 22116 author Bouras Constantin 27275 author Savioz Armand 0000-0001-8059-8281 135243 322-330 10.1016/j.neurobiolaging.2013.08.005 24011540 0197-4580 Superficial layers I to III of the human cerebral cortex are more vulnerable toward Aβ peptides than deep layers V to VI in aging. Three models of layers were used to investigate this pattern of frailty. First, primary neurons from E14 and E17 embryonic murine cortices, corresponding respectively to future deep and superficial layers, were treated either with Aβ1-42, okadaic acid, or kainic acid. Second, whole E14 and E17 embryonic cortices, and third, in vitro separated deep and superficial layers of young and old C57BL/6J mice, were treated identically. We observed that E14 and E17 neurons in culture were prone to death after the Aβ and particularly the kainic acid treatment. This was also the case for the superficial layers of the aged cortex, but not for the embryonic, the young cortex, and the deep layers of the aged cortex. Thus, the aged superficial layers appeared to be preferentially vulnerable against Aβ and kainic acid. This pattern of vulnerability corresponds to enhanced accumulation of senile plaques in the superficial cortical layers with aging and Alzheimer's disease. Abeta amyloid Aging Alzheimer's disease Cell viability Cell death Cerebral cortex Cortical layers Cortical vulnerability Cux2 Kainic acid Okadaic acid PSD-95 Dewey 616.89 60 Département de psychiatrie 225 false Neurobiology of aging 35 2 d91fc9d0-d98b-420f-8e63-ff9ee65739e8 Article (Published version) Restricted 1-s2.0-S0197458013003394-main.pdf 1830301 application/pdf restrictedAccess Faculté de médecine / Section de médecine clinique / Département de psychiatrie

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A comparison of frailty of primary neurons, embryonic, and aging mouse cortical layers	Neurobiology of aging			2014	769	2

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Fugistier, Patrick