Scientific Article
previous document  unige:30566  next document
add to browser collection
Title

AnoEST: toward A. gambiae functional genomics

Authors
Koutsos, Anastasios C
Blass, Claudia
Kafatos, Fotis C
Christophides, George K
Published in Genome Research. 2005, vol. 15, no. 6, p. 893-9
Abstract Here, we present an analysis of 215,634 EST and cDNA sequences of a major vector of human malaria Anopheles gambiae structured into the AnoEST database. The expressed sequences are grouped into clusters using genomic sequence as template and associated with inferred functional annotation, including the following: corresponding Ensembl gene prediction, putative orthologous genes in other species, homology to known proteins, protein domains, associated Gene Ontology terms, and corresponding classification into broad GO-slim functional groups. AnoEST is a vital resource for interpretation of expression profiles derived using recently developed A. gambiae cDNA microarrays. Using these cDNA microarrays, we have experimentally confirmed the expression of 7961 clusters during mosquito development. Of these, 3100 are not associated with currently predicted genes. Moreover, we found that clusters with confirmed expression are nonbiased with respect to the current gene annotation or homology to known proteins. Consequently, we expect that many as yet unconfirmed clusters are likely to be actual A. gambiae genes. [AnoEST is publicly available at http://komar.embl.de, and is also accessible as a Distributed Annotation Service (DAS).].
Keywords AnimalsAnopheles gambiae/embryology/geneticsDatabases, GeneticExpressed Sequence TagsGene Expression Regulation, DevelopmentalGenes, InsectGenome
Identifiers
PMID: 15899967
Full text
Article (Published version) (610 Kb) - public document Free access
Citation
(ISO format)
KRIVENTSEVA, Evgenia et al. AnoEST: toward A. gambiae functional genomics. In: Genome Research, 2005, vol. 15, n° 6, p. 893-9. https://archive-ouverte.unige.ch/unige:30566

192 hits

137 downloads

Update

Deposited on : 2013-10-22

Export document
Format :
Citation style :