Scientific article
Open access

AnoEST: toward A. gambiae functional genomics

Published inGenome research, vol. 15, no. 6, p. 893-899
Publication date2005

Here, we present an analysis of 215,634 EST and cDNA sequences of a major vector of human malaria Anopheles gambiae structured into the AnoEST database. The expressed sequences are grouped into clusters using genomic sequence as template and associated with inferred functional annotation, including the following: corresponding Ensembl gene prediction, putative orthologous genes in other species, homology to known proteins, protein domains, associated Gene Ontology terms, and corresponding classification into broad GO-slim functional groups. AnoEST is a vital resource for interpretation of expression profiles derived using recently developed A. gambiae cDNA microarrays. Using these cDNA microarrays, we have experimentally confirmed the expression of 7961 clusters during mosquito development. Of these, 3100 are not associated with currently predicted genes. Moreover, we found that clusters with confirmed expression are nonbiased with respect to the current gene annotation or homology to known proteins. Consequently, we expect that many as yet unconfirmed clusters are likely to be actual A. gambiae genes. [AnoEST is publicly available at http://komar.embl.de, and is also accessible as a Distributed Annotation Service (DAS).].

  • Animals
  • Anopheles gambiae/embryology/genetics
  • Databases, Genetic
  • Expressed Sequence Tags
  • Gene Expression Regulation, Developmental
  • Genes, Insect
  • Genome
Affiliation Not a UNIGE publication
Citation (ISO format)
KRIVENTSEVA, Evgenia et al. AnoEST: toward A. gambiae functional genomics. In: Genome research, 2005, vol. 15, n° 6, p. 893–899. doi: 10.1101/gr.3756405
Main files (1)
Article (Published version)
ISSN of the journal1088-9051

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