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Calcium measurements in organelles with Ca2+-sensitive fluorescent proteins

Published in Cell Calcium. 2005, vol. 38, no. 3-4, p. 213-22
Abstract The recent improvement in the design and use of genetically encoded fluorescent Ca2+ indicators should foster major progress in three aspects of Ca2+ signaling. At the subcellular level, ratiometric probes with expanded dynamics are now available to measure accurately the local Ca2+ changes occurring within specific cell compartments. These tools will allow to determine precisely the role of organelles and of cellular microdomains in Ca2+ handling. At the cellular level, the permanent labeling offered by the genetic probes enables large-scale, long-term Ca2+ measurements with robotic multiplexing technologies such as fluorescence plate readers or automated microscopes. This opens the way to large-scale pharmacological or genetic screens based on organelle-specific functional assays. At the whole animal level, probes with improved dynamics and reduced interference with endogenous proteins will allow to generate transgenic animals bearing Ca2+ sensitive indicators in specific cells and tissues. With this approach, Ca2+ signals can be recorded in neurons, heart, and pancreas of live animals during physiological and pathological stimulations. In this chapter, I will review the progress made in the design and use of genetic Ca2+ indicators, and discuss how these new tools provide an opportunity to challenge several unsolved questions in Ca2+ signaling.
Keywords AnimalsCalcium/analysis/chemistry/physiologyGreen Fluorescent Proteins/chemistry/genetics/metabolismHumansOrganelles/chemistry/genetics
PMID: 16122797
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Research group Signaux intracellulaires (210)
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DEMAUREX, Nicolas. Calcium measurements in organelles with Ca2+-sensitive fluorescent proteins. In: Cell Calcium, 2005, vol. 38, n° 3-4, p. 213-22. https://archive-ouverte.unige.ch/unige:30418

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Deposited on : 2013-10-16

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