Ultraviolet-B-mediated induction of protein-protein interactions in mammalian cells

Crefcoeur, Remco Paul; Yin, Ruohe; Ulm, Roman; Halazonetis, Thanos

doi:10.1038/ncomms2800

Scientific article

English

Ultraviolet-B-mediated induction of protein-protein interactions in mammalian cells

Contributors

Crefcoeur, Remco Paul; Yin, Ruohe; Ulm, Roman

; Halazonetis, Thanos

Published in

Nature communications, vol. 4, 1779

Publication date

2013

Abstract

Light-sensitive proteins are useful tools to control protein localization, activation and gene expression, but are currently limited to excitation with red or blue light. Here we report a novel optogenetic system based on the ultraviolet-B-dependent interaction of the Arabidopsis ultraviolet-B photoreceptor UVR8 with COP1 that can be performed in visible light background. We use this system to induce nuclear accumulation of cytoplasmic green fluorescent protein fused to UVR8 in cells expressing nuclear COP1, and to recruit a nucleoplasmic red fluorescent protein fused to COP1 to chromatin in cells expressing UVR8-H2B. We also show that ultraviolet-B-dependent interactions between DNA-binding and transcription activation domains result in a linear induction of gene expression. The UVR8-COP1 interactions in mammalian cells can be induced using subsecond pulses of ultraviolet-B light and last several hours. As UVR8 photoperception is based on intrinsic tryptophan residues, these interactions do not depend on the addition of an exogenous chromophore.

UNIGE affiliation entities

Citation (ISO format)

CREFCOEUR, Remco Paul et al. Ultraviolet-B-mediated induction of protein-protein interactions in mammalian cells. In: Nature communications, 2013, vol. 4, p. 1779. doi: 10.1038/ncomms2800

Article (Published version)

Identifiers

PID : unige:29083
DOI : 10.1038/ncomms2800
PMID : 23653191

Additional URL for this publication

http://www.nature.com/ncomms/journal/v4/n4/full/ncomms2800.html

Journal ISSN

2041-1723

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Creation

06/08/2013 10:42:00

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06/08/2013 10:42:00

Update

14/03/2023 20:21:40

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14/03/2023 20:21:40

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30/10/2024 09:52:27

Ultraviolet-B-mediated induction of protein-protein interactions in mammalian cells

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Published in

Publication date

Abstract

UNIGE affiliation entities

Citation (ISO format)

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