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Title

Human papillomavirus (HPV) DNA triage of women with atypical squamous cells of undetermined significance with cobas 4800 HPV and Hybrid Capture 2 tests for detection of high-grade lesions of the uterine cervix

Authors
Lapierre, Simon Grandjean
Sauthier, Philippe
Mayrand, Marie-Hélène
Dufresne, Simon
Provencher, Diane
Drouin, Pierre
Gauthier, Philippe
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Published in Journal of Clinical Microbiology. 2012, vol. 50, no. 4, p. 1240-4
Abstract The triage of women with high-risk (HR) human papillomavirus (HPV)-positive smears for atypical squamous cells of undetermined significance (ASC-US) to colposcopy is now an integrated option in clinical guidelines. The performance of cobas 4800 HPV and that of Hybrid Capture 2 (HC2) for HR HPV DNA detection in cervical samples in PreservCyt were compared in 396 women referred to colposcopy for ASC-US. Of these, 316 did not have cervical intraepithelial neoplasia (CIN), 47 had CIN1, 29 had CIN2 or CIN3 (CIN2+), and 4 had CIN of unknown grade. HR HPV was detected in 129 (32.6%) and 149 (37.6%) samples with HC2 and cobas 4800 HPV, respectively (P = 0.15). The clinical sensitivities and specificities for detecting CIN2+ were 89.7% (95% confidence interval [CI], 72.8 to 97.2%) and 66.7% (95% CI, 61.7 to 71.3%) with cobas 4800 HPV and 93.1% (95% CI, 77.0 to 99.2%) and 72.2% (95% CI 67.4 to 76.5%) with HC2. The performance of cobas 4800 HPV was similar to that of HC2 for identifying women with ASC-US who would benefit the most from colposcopy.
Keywords AdultAgedCervical Intraepithelial Neoplasia/diagnosis/pathology/virologyColposcopyDNA, ViralEpithelium/pathologyFemaleGenotypeHuman papillomavirus 16/geneticsHuman papillomavirus 18/geneticsHumansMiddle AgedMolecular Diagnostic TechniquesPapillomavirus Infections/diagnosis/pathology/virologySensitivity and SpecificityUterine Cervical Neoplasms/diagnosis/pathology/virologyYoung Adult
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PMID: 22301023
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Research group GYNOB DEPARTEMENT (508)
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LAPIERRE, Simon Grandjean et al. Human papillomavirus (HPV) DNA triage of women with atypical squamous cells of undetermined significance with cobas 4800 HPV and Hybrid Capture 2 tests for detection of high-grade lesions of the uterine cervix. In: Journal of Clinical Microbiology, 2012, vol. 50, n° 4, p. 1240-4. https://archive-ouverte.unige.ch/unige:28809

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Deposited on : 2013-07-08

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