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Scientific article
English

In vivo reprogramming of circuit connectivity in postmitotic neocortical neurons

Contributors

De La Rossa, Andrés; Bellone, Camilla; Golding, Bruno James; Vitali, Ilaria; Moss, Jonathan; Toni, Nicolas; Luscher, Christian; Jabaudon, Denis

Published in

Nature neuroscience, vol. 16, no. 2, p. 193-200

Publication date

2013

Abstract

The molecular mechanisms that control how progenitors generate distinct subtypes of neurons, and how undifferentiated neurons acquire their specific identity during corticogenesis, are increasingly understood. However, whether postmitotic neurons can change their identity at late stages of differentiation remains unknown. To study this question, we developed an electrochemical in vivo gene delivery method to rapidly manipulate gene expression specifically in postmitotic neurons. Using this approach, we found that the molecular identity, morphology, physiology and functional input-output connectivity of layer 4 mouse spiny neurons could be specifically reprogrammed during the first postnatal week by ectopic expression of the layer 5B output neuron-specific transcription factor Fezf2. These findings reveal a high degree of plasticity in the identity of postmitotic neocortical neurons and provide a proof of principle for postnatal re-engineering of specific neural microcircuits in vivo.

Keywords

  • Age Factors
  • Animals
  • Animals, Newborn
  • Cell Cycle/drug effects/genetics
  • Cholera Toxin/metabolism
  • Cyclohexanones/pharmacology
  • DNA-Binding Proteins/genetics/metabolism
  • Dendrites/metabolism
  • Embryo, Mammalian
  • Epithelial Sodium Channels/genetics
  • Gene Expression Regulation, Developmental/drug effects/genetics/physiology
  • Green Fluorescent Proteins/genetics
  • Membrane Potentials/drug effects/genetics
  • Mice
  • Mice, Transgenic
  • Microscopy, Electron, Transmission
  • Neocortex/cytology/embryology/growth & development
  • Nerve Net/physiology
  • Nerve Tissue Proteins/genetics/metabolism
  • Neural Pathways/physiology
  • Neurons/physiology/ultrastructure
  • Patch-Clamp Techniques
  • Rhodopsin/genetics
  • Statistics, Nonparametric
  • Vesicular Glutamate Transport Protein 2/metabolism

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

DE LA ROSSA, Andrés et al. In vivo reprogramming of circuit connectivity in postmitotic neocortical neurons. In: Nature neuroscience, 2013, vol. 16, n° 2, p. 193–200. doi: 10.1038/nn.3299

Main files (1)

Article (Published version)

Identifiers

Journal ISSN

1097-6256
770views
6downloads

Technical informations

Creation

30/05/2013 11:51:00

First validation

30/05/2013 11:51:00

Update

13/10/2025 23:30:51

Status update

14/03/2023 20:19:21

Last indexation

03/11/2025 16:23:10
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