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Scientific article
English

Detection of miRNA in Live Cells by Using Templated RuII-Catalyzed Unmasking of a Fluorophore

Published inChemistry, vol. 19, no. 25, p. 8182-8189
Publication date2013
Abstract

Reactions templated by cellular nucleic acids are attractive for nucleic acid sensing or responsive systems. Herein we report the use of a photocatalyzed reductive cleavage of an immolative linker to unmask a rhodamine fluorophore, and its application to miRNA imaging. The reaction was found to proceed with a very high turnover (>4000) and provided reliable detection down to 5 pm of template by using γ-serine-modified peptide nucleic acid (PNA) probes. The reaction was used for the selective detection of miR-21 in BT474 cells and miR-31 in HeLa cells following irradiation for 30 min. The probes were introduced by using reversible permeation with streptolysin-O (SLO) or a transfection technique.

Keywords
  • bioorthogonal chemistry
  • imaging agents
  • peptides
  • RNA recognition
  • templated reactions
Citation (ISO format)
SADHU, Kalyan K., WINSSINGER, Nicolas. Detection of miRNA in Live Cells by Using Templated Ru<sup>II</sup>-Catalyzed Unmasking of a Fluorophore. In: Chemistry, 2013, vol. 19, n° 25, p. 8182–8189. doi: 10.1002/chem.201300060
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Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal0947-6539
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Technical informations

Creation06/10/2013 10:27:00 AM
First validation06/10/2013 10:27:00 AM
Update time03/14/2023 8:18:50 PM
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