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Titles listDetection of miRNA in Live Cells by Using Templated RuII-Catalyzed Unmasking of a Fluorophore
Titles listDetection of miRNA in Live Cells by Using Templated RuII-Catalyzed Unmasking of a Fluorophore
Scientific Article
unige:28609 
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Detection of miRNA in Live Cells by Using Templated RuII-Catalyzed Unmasking of a Fluorophore |
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| Published in | Chemistry - A European Journal. 2013, vol. 19, no. 25, p. 8182-8189 | |
| Abstract | Reactions templated by cellular nucleic acids are attractive for nucleic acid sensing or responsive systems. Herein we report the use of a photocatalyzed reductive cleavage of an immolative linker to unmask a rhodamine fluorophore, and its application to miRNA imaging. The reaction was found to proceed with a very high turnover (>4000) and provided reliable detection down to 5 pm of template by using γ-serine-modified peptide nucleic acid (PNA) probes. The reaction was used for the selective detection of miR-21 in BT474 cells and miR-31 in HeLa cells following irradiation for 30 min. The probes were introduced by using reversible permeation with streptolysin-O (SLO) or a transfection technique. | |
| Keywords | bioorthogonal chemistry — imaging agents — peptides — RNA recognition — templated reactions | |
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| Citation (ISO format) | SADHU, Kalyan K., WINSSINGER, Nicolas. Detection of miRNA in Live Cells by Using Templated RuII-Catalyzed Unmasking of a Fluorophore. In: Chemistry - A European Journal, 2013, vol. 19, n° 25, p. 8182-8189. https://archive-ouverte.unige.ch/unige:28609 |
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