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Detection of miRNA in Live Cells by Using Templated RuII-Catalyzed Unmasking of a Fluorophore

Sadhu, Kalyan K.
Published in Chemistry - A European Journal. 2013, vol. 19, no. 25, p. 8182-8189
Abstract Reactions templated by cellular nucleic acids are attractive for nucleic acid sensing or responsive systems. Herein we report the use of a photocatalyzed reductive cleavage of an immolative linker to unmask a rhodamine fluorophore, and its application to miRNA imaging. The reaction was found to proceed with a very high turnover (>4000) and provided reliable detection down to 5 pm of template by using γ-serine-modified peptide nucleic acid (PNA) probes. The reaction was used for the selective detection of miR-21 in BT474 cells and miR-31 in HeLa cells following irradiation for 30 min. The probes were introduced by using reversible permeation with streptolysin-O (SLO) or a transfection technique.
Keywords bioorthogonal chemistryimaging agentspeptidesRNA recognitiontemplated reactions
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Other version: http://doi.wiley.com/10.1002/chem.201300060
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SADHU, Kalyan K., WINSSINGER, Nicolas. Detection of miRNA in Live Cells by Using Templated RuII-Catalyzed Unmasking of a Fluorophore. In: Chemistry - A European Journal, 2013, vol. 19, n° 25, p. 8182-8189. https://archive-ouverte.unige.ch/unige:28609

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Deposited on : 2013-06-17

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