In vivo assessment of myelination by phase imaging at high magnetic field

Lodygensky, Gregory Anton; Marques, José P; Maddage, Rajika; Perroud, Elodie Nathalie; Sizonenko, Stéphane; Hüppi, Petra Susan; Gruetter, Rolf

doi:10.1016/j.neuroimage.2011.09.057

Scientific article

English

In vivo assessment of myelination by phase imaging at high magnetic field

Contributors

Lodygensky, Gregory Anton; Marques, José P; Maddage, Rajika; Perroud, Elodie Nathalie; Sizonenko, Stéphane

; Hüppi, Petra Susan; Gruetter, Rolf

Published in

NeuroImage, vol. 59, no. 3, p. 1979-1987

Publication date

2012

Abstract

The present study evaluated the potential of using the phase of T2* weighted MR images to characterize myelination during brain development and pathology in rodents at 9.4 T. Phase contrast correlated with myelin content assessed by histology and suggests that most contrast between white and cortical gray matter is modulated by myelin. Ex vivo experiments showed that gray-white matter phase contrast remains unchanged after iron extraction. In dysmyelinated shiverer mice, phase imaging correlated strongly with myelin staining, showing reduced contrast between white and gray matter when compared to healthy controls. We conclude that high-resolution phase images, acquired at high field, allow assessment of myelination and dysmyelination.

Keywords

Animals
Brain Chemistry/physiology
Coloring Agents
Corpus Callosum/anatomy & histology/physiology
Demyelinating Diseases/pathology
Densitometry
Electromagnetic Fields
Image Processing, Computer-Assisted
Iron/metabolism
Magnetic Resonance Imaging/methods
Male
Mice
Mice, Inbred C3H
Mice, Neurologic Mutants
Microscopy, Phase-Contrast/methods
Myelin Sheath/physiology
Rats
Rats, Wistar

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UNIGE research groups

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Citation (ISO format)

LODYGENSKY, Gregory Anton et al. In vivo assessment of myelination by phase imaging at high magnetic field. In: NeuroImage, 2012, vol. 59, n° 3, p. 1979–1987. doi: 10.1016/j.neuroimage.2011.09.057

Article (Published version)

Identifiers

PID : unige:28596
DOI : 10.1016/j.neuroimage.2011.09.057
PMID : 21985911

Journal ISSN

1053-8119

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In vivo assessment of myelination by phase imaging at high magnetic field

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Abstract

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UNIGE research groups

Citation (ISO format)

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