UNIGE document Scientific Article
previous document  unige:28552  next document
add to browser collection
Title

Setting up and monitoring an infection of Dictyostelium discoideum with mycobacteria

Authors
Andreu, Nuria
Wiles, Siouxsie
Robertson, Brian
Published in Methods in Molecular Biology. 2013, vol. 983, p. 403-17
Abstract Mycobacterium marinum is the causative agent of fish and amphibian tuberculosis in the wild. It is a genetically close cousin of Mycobacterium tuberculosis, and thereby the infection process remarkably shares many of the hallmarks of M. tuberculosis infection in human, at both the cellular and organism levels. Therefore, M. marinum is used as a model for the study of mycobacterial infection in various host organisms. Recently, the Dictyostelium-M. marinum system has been shown to be a valuable model that recapitulates the main features of the intracellular fate of M. marinum including phagosome maturation arrest, as well as its particular cell-to-cell dissemination mode. We present here a "starter kit" of detailed methods that allows to establish an infection of Dictyostelium with M. marinum and to monitor quantitatively the intracellular bacterial growth.
Identifiers
PMID: 23494320
Full text
Article (Published version) (617 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Citation
(ISO format)
ARAFAH, Sonia et al. Setting up and monitoring an infection of Dictyostelium discoideum with mycobacteria. In: Methods in Molecular Biology, 2013, vol. 983, p. 403-17. https://archive-ouverte.unige.ch/unige:28552

185 hits

0 download

Update

Deposited on : 2013-06-12

Export document
Format :
Citation style :