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Scientific article
English

Selective affinity-based probe for oncogenic kinases suitable for live cell imaging

Published inChemical science, vol. 4, no. 5, p. 2088-2092
Publication date2013
Abstract

Cell permeable probes to image the presence and localization of kinases are important in studying their function and as diagnostic tools. Despite the central role of kinases as therapeutic targets, there are remarkably few probes available. Herein we report the discovery of a probe to image two therapeutically relevant kinases: EGFR and ERBB2. The probe was identified from a library based on a scaffold derived from the resorcyclic acid lactones that form a covalent adduct by reacting specifically with an unconserved cysteine in the nucleotide-binding site of the kinases. We demonstrated the utility of the newly discovered probe by imaging of EGFR localization and ERBB2 inhibition in live cells.

Citation (ISO format)
ZAMBALDO, Claudio et al. Selective affinity-based probe for oncogenic kinases suitable for live cell imaging. In: Chemical science, 2013, vol. 4, n° 5, p. 2088–2092. doi: 10.1039/c3sc21856b
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ISSN of the journal2041-6520
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Technical informations

Creation04/08/2013 12:04:00 PM
First validation04/08/2013 12:04:00 PM
Update time03/14/2023 8:08:04 PM
Status update03/14/2023 8:08:04 PM
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