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Scientific article
English

EasyProt - An easy-to-use graphical platform for proteomics data analysis

Published inJournal of proteomics, vol. 79, p. 146-160
Publication date2013
Abstract

High throughput protein identification and quantification analysis based on mass spectrometry are fundamental steps in most proteomics projects. Here, we present EasyProt (available at http://easyprot.unige.ch), a new platform for mass spectrometry data processing, protein identification, quantification and unexpected post-translational modification characterization. EasyProt provides a fully integrated graphical experience to perform a large part of the proteomic data analysis workflow. Our goal was to develop a software platform that would fulfill the needs of scientists in the field, while emphasizing ease-of-use for non-bioinformatician users. Protein identification is based on OLAV scoring schemes and protein quantification is implemented for both, isobaric labeling and label-free methods. Additional features are available, such as peak list processing, isotopic correction, spectra filtering, charge-state deconvolution and spectra merging. To illustrate the EasyProt platform, we present two identification and quantification workflows based on isobaric tagging and label-free methods.

Keywords
  • Protein identification
  • Protein quantification
  • Label-free
  • Isobaric labeling
  • Post-translational modifications
  • Mass spectrometry
Citation (ISO format)
GLUCK, Florent et al. EasyProt - An easy-to-use graphical platform for proteomics data analysis. In: Journal of proteomics, 2013, vol. 79, p. 146–160. doi: 10.1016/j.jprot.2012.12.012
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ISSN of the journal1874-3919
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