A simplified method for busulfan monitoring using dried blood spot in combination with liquid chromatography/tandem mass spectrometry

Ansari Djaberi, Marc Georges; Uppugunduri Satyanarayana, Chakradhara Rao; Deglon, Julien; Théorêt, Yves; Versace, François; Gumy Pause, Fabienne; Ozsahin, Ayse Hulya; Dayer, Pierre; Desmeules, Jules Alexandre; Daali, Youssef

doi:10.1002/rcm.6241

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Title		A simplified method for busulfan monitoring using dried blood spot in combination with liquid chromatography/tandem mass spectrometry
Authors		Ansari Djaberi, Marc Georges Uppugunduri Satyanarayana, Chakradhara Rao Deglon, Julien Théorêt, Yves Versace, François Gumy Pause, Fabienne Ozsahin, Ayse Hulya Dayer, Pierre Desmeules, Jules Alexandre Daali, Youssef show all authors [1 - 10]
Published in		Rapid Communications in Mass Spectrometry. 2012, vol. 26, no. 12, p. 1437-46
Abstract		Busulfan (Bu) is an important component of the myeloablative conditioning regimen prior to hematopoietic stem cell transplantation (HSCT) especially in children. Intravenously administered Bu exhibits a therapeutic window phenomenon requiring therapeutic drug monitoring. Analytical methods developed for Bu routine monitoring were aimed at using low volumes of biological fluids and development of simple procedures to facilitate the dosage adjustment. In this report, we describe a simple, rapid method for Bu measurement using dried blood spots (DBS) from only 5 μL of whole blood.
Keywords		Busulfan/blood/pharmacokinetics — Calibration — Child — Chromatography, High Pressure Liquid/methods — Dried Blood Spot Testing/methods — Drug Stability — Humans — Linear Models — Reproducibility of Results — Spectrometry, Mass, Electrospray Ionization — Tandem Mass Spectrometry/methods
Identifiers		DOI: 10.1002/rcm.6241 PMID: 22592987
Full text		Article (Published version) (768 Kb) - Free access
Structures		Faculté de médecine / Section de médecine clinique / Département d'anesthésiologie, pharmacologie, soins intensifs et urgences Faculté de médecine / Section de médecine clinique / Département de pédiatrie, gynécologie et obstétrique
Research groups		Pharmaco-omiques et médecine de précision (1003) Groupe Desmeules Jules (pharmacologie/toxicologie) (567) Groupe Ozsahin Ayse Hulya (onco-hémato pédiatrie) (889) Plateforme d'oncologie et d'hématologie pédiatrique (907)
Citation (ISO format)		ANSARI DJABERI, Marc Georges et al. A simplified method for busulfan monitoring using dried blood spot in combination with liquid chromatography/tandem mass spectrometry. In: Rapid Communications in Mass Spectrometry, 2012, vol. 26, n° 12, p. 1437-46. doi: 10.1002/rcm.6241 https://archive-ouverte.unige.ch/unige:26868