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Scientific article
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English

A simplified method for busulfan monitoring using dried blood spot in combination with liquid chromatography/tandem mass spectrometry

Published inRCM. Rapid communications in mass spectrometry, vol. 26, no. 12, p. 1437-1446
Publication date2012
Abstract

Busulfan (Bu) is an important component of the myeloablative conditioning regimen prior to hematopoietic stem cell transplantation (HSCT) especially in children. Intravenously administered Bu exhibits a therapeutic window phenomenon requiring therapeutic drug monitoring. Analytical methods developed for Bu routine monitoring were aimed at using low volumes of biological fluids and development of simple procedures to facilitate the dosage adjustment. In this report, we describe a simple, rapid method for Bu measurement using dried blood spots (DBS) from only 5 μL of whole blood.

Keywords
  • Busulfan/blood/pharmacokinetics
  • Calibration
  • Child
  • Chromatography, High Pressure Liquid/methods
  • Dried Blood Spot Testing/methods
  • Drug Stability
  • Humans
  • Linear Models
  • Reproducibility of Results
  • Spectrometry, Mass, Electrospray Ionization
  • Tandem Mass Spectrometry/methods
Citation (ISO format)
ANSARI DJABERI, Marc Georges et al. A simplified method for busulfan monitoring using dried blood spot in combination with liquid chromatography/tandem mass spectrometry. In: RCM. Rapid communications in mass spectrometry, 2012, vol. 26, n° 12, p. 1437–1446. doi: 10.1002/rcm.6241
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Article (Published version)
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Identifiers
ISSN of the journal0951-4198
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