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Scientific article
English

Biosensor based on chemically-designed anchorable cytochrome c for the detection of H2O2 released by aquatic cells

Contributors

Suárez, Guillaume; Santschi, Christian; Martin, Olivier J.F.; Slaveykova, Vera

Published in

Biosensors & bioelectronics, vol. 42, p. 385-390

Publication date

2013

Abstract

A novel third generation biosensor was developed based on one-shot adsorption of chemically-modified cytochrome c (cyt c) onto bare gold electrodes. In contrast to the classic approach which consists of attaching cyt c onto an active self-assembled monolayer (SAM) priory chemisorbed on gold, here short-chain thiol derivatives (mercaptopropionic acid, MPA) were chemically introduced on cyt c protein shell via its lysine residues enabling the very fast formation (<5 min) of an electroactive biological self-assembled monolayer (SAM) exhibiting a quasi-reversible electrochemical behavior and a fast direct electron transfer (ET). The heterogeneous ET rate constant was estimated to be ks=1600 s−1, confirming that short anchors facilitate the ET via an efficient orientation of the heme pocket. In comparison, no ET was observed in the case of native and long-anchor (mercaptoundecanoic acid, MUA) modified cyt c directly adsorbed on gold. However, in both cases the ET was efficiently restored upon in-bulk generation of gold nanoparticles which acted as electron shuttles. This observation emphasizes that the lack of electroactivity might be caused by either an inappropriate orientation of the protein (native cyt c) or a critical distance (MUA-cyt c). Finally, the sensitivity of the bare gold electrode directly modified with MPA-cyt c to hydrogen peroxide (H2O2) was evaluated by amperometry and the so-made amperometric biosensor was able to perform real-time and non-invasive detection of endogeneous H2O2 released by unicellular aquatic microorganisms, Chlamydomonas reinhardtii, upon cadmium exposure.

Keywords

  • Cytochrome c
  • Bioelectrochemistry
  • Direct electron transfer
  • Chemical manipulation
  • Oxidative stress
  • Aquatic cells

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

SUÁREZ, Guillaume et al. Biosensor based on chemically-designed anchorable cytochrome c for the detection of H2O2 released by aquatic cells. In: Biosensors & bioelectronics, 2013, vol. 42, p. 385–390. doi: 10.1016/j.bios.2012.10.083

Main files (1)

Article (Published version)

Identifiers

Additional URL for this publication

http://linkinghub.elsevier.com/retrieve/pii/S0956566312007774

Journal ISSN

0956-5663
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Technical informations

Creation

12/03/2013 18:55:00

First validation

12/03/2013 18:55:00

Update

14/03/2023 20:05:13

Status update

14/03/2023 20:05:12

Last indexation

30/10/2024 08:59:51
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