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Identification of a small molecule yeast TORC1 inhibitor with a multiplex screen based on flow cytometry

Chen, Jun
Young, Susan M
Allen, Chris
Péli-Gulli, Marie-Pierre
Panchaud, Nicolas
Waller, Anna
Ursu, Oleg
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Published in ACS Chemical Biology. 2012, vol. 7, no. 4, p. 715-22
Abstract TOR (target of rapamycin) is a serine/threonine kinase, evolutionarily conserved from yeast to human, which functions as a fundamental controller of cell growth. The moderate clinical benefit of rapamycin in mTOR-based therapy of many cancers favors the development of new TOR inhibitors. Here we report a high-throughput flow cytometry multiplexed screen using five GFP-tagged yeast clones that represent the readouts of four branches of the TORC1 signaling pathway in budding yeast. Each GFP-tagged clone was differentially color-coded, and the GFP signal of each clone was measured simultaneously by flow cytometry, which allows rapid prioritization of compounds that likely act through direct modulation of TORC1 or proximal signaling components. A total of 255 compounds were confirmed in dose-response analysis to alter GFP expression in one or more clones. To validate the concept of the high-throughput screen, we have characterized CID 3528206, a small molecule most likely to act on TORC1 as it alters GFP expression in all five GFP clones in a manner analogous to that of rapamycin. We have shown that CID 3528206 inhibited yeast cell growth and that CID 3528206 inhibited TORC1 activity both in vitro and in vivo with EC(50)'s of 150 nM and 3.9 μM, respectively. The results of microarray analysis and yeast GFP collection screen further support the notion that CID 3528206 and rapamycin modulate similar cellular pathways. Together, these results indicate that the HTS has identified a potentially useful small molecule for further development of TOR inhibitors.
Keywords Flow CytometryGreen Fluorescent ProteinsHumansProtein Kinase Inhibitors/analysisSaccharomyces cerevisiae/drug effectsSaccharomyces cerevisiae Proteins/antagonists & inhibitorsSignal Transduction/drug effectsTranscription Factors/antagonists & inhibitors
PMID: 22260433
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Project FNS: Frontiers in Genetics
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CHEN, Jun et al. Identification of a small molecule yeast TORC1 inhibitor with a multiplex screen based on flow cytometry. In: ACS Chemical Biology, 2012, vol. 7, n° 4, p. 715-22. doi: 10.1021/cb200452r https://archive-ouverte.unige.ch/unige:26555

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Deposited on : 2013-03-04

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