Upon entry of HIV-1 in the target cell, the viral RNA genome contained in a conical capsid (CA) shell is reverse transcribed into its DNA complement (cDNA), stimulating disassembly of the CA core. The resulting pre-integration complex (PIC), minimally bearing integrase (IN) protein and the viral DNA, accesses the nucleus of the target cell where the cDNA integrates into the host chromosomes in order to replicate. Genetic experiments indicate that CA is critically important for these early steps in the infection cycle that culminate in integration. HIV-1 interacts with host factors to hijack cellular functions for a successful infection. At the same time, the host cell developed systems to prevent efficient replication of HIV-1. In this work we investigated the role of three host factors regulating HIV-1 replication in a CA-dependent manner: transportin SR-2 (TNPO3), cleavage and polyadenylation specific factor 6 (CPSF6) and cyclophilin A (CypA).