UNIGE document Scientific Article
previous document  unige:26035  next document
add to browser collection
Title

Immunogenicity of HIV type 1 gp120 CD4 binding site phage mimotopes

Authors
Dorgham, Karim
Dogan, Ismaïl
Bitton, Natacha
Parizot, Christophe
Debré, Patrice
Gorochov, Guy
Published in AIDS Research and Human Retroviruses. 2005, vol. 21, no. 1, p. 82-92
Abstract The conserved domain of the CD4 binding site (CD4bs) on the human immunodeficiency virus type 1 (HIV- 1) envelope represents a potential target for vaccine development. Here we describe selection of peptide mimotopes by panning a phage peptide library on the HIV-1 CD4bs-specific, broadly neutralizing anti-HIV-1 monoclonal antibody, IgG(1) b12. We identified an initial consensus sequence for IgG1 b12 binding (M/VThetaSD, where Theta represents an aromatic amino acid). A molecular evolution approach, using second- and third-generation libraries, led us to identify a refined consensus sequence (GLLVWSDEL). The resulting IgG1 b12 phage mimotopes compete with gp160 for the IgG1 b12 antigen-binding site, but the phage coat protein (pIII) may play an important structural role, since both free peptides and KLH-conjugated peptides have no detectable binding activity. Mice immunized with IgG1 b12 phage mimotopes elicited a weak but persistent humoral response directed against the HIV-1 envelope. An antibody fragment was isolated from the antibody repertoires of these animals. It is noteworthy that while it has a relatively low affinity for HIV-1 gp160, the antibody targets an epitope that overlaps with that of IgG1 b12. Our data therefore suggest that engineered IgG1 b12 mimotopes share immunogenic features with the CD4bs. However, these peptidic structures will require further improvement in order to generate broad specificity neutralizing antibodies like IgG1 b12.
Keywords Amino Acid SequenceAnimalsAntibodiesMonoclonal/immunologyAntigensCD4/metabolismBacteriophages/geneticsBase SequenceBinding SitesFemaleHIV Antibodies/bloodHIV Envelope Protein gp120/chemistry/immunology/metabolismHumansImmunizationImmunoglobulin G/immunologyMiceMiceInbred C57BLMolecular Sequence DataPeptide LibraryPeptides/chemical synthesis/chemistry/immunology
Identifiers
PMID: 15665647
Full text
Article (Published version) (266 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Research group HIV (835)
Citation
(ISO format)
DORGHAM, Karim et al. Immunogenicity of HIV type 1 gp120 CD4 binding site phage mimotopes. In: AIDS Research and Human Retroviruses, 2005, vol. 21, n° 1, p. 82-92. https://archive-ouverte.unige.ch/unige:26035

216 hits

0 download

Update

Deposited on : 2013-01-30

Export document
Format :
Citation style :