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Title

Comparative analysis of PCR-electrospray ionization/mass spectrometry (MS) and MALDI-TOF/MS for the identification of bacteria and yeast from positive blood culture bottles

Authors
Kaleta, Erin J
Clark, Andrew E
Wysocki, Vicki H
Ingram, Elizabeth L
Wolk, Donna M
Published in Clinical Chemistry. 2011, vol. 57, no. 7, p. 1057-67
Abstract Emerging technologies for rapid identification of microbes demonstrate a shift from traditional biochemical and molecular testing algorithms toward methods using mass spectrometry (MS) for the semiquantitative analysis of microbial proteins and genetic elements. This study was performed to assess the diagnostic accuracy of 2 such technologies, PCR-electrospray ionization (ESI)/MS and MALDI-TOF/MS, with respect to phenotypic and biochemical profiling as a reference standard method. A positive challenge set of blood culture bottles was used to compare PCR-ESI/MS and MALDI-TOF/MS performance on a matched set of samples.
Keywords Bacteremia/blood/diagnosis/microbiologyBacteria/classification/genetics/isolation & purificationBase SequenceDNA, Bacterial/geneticsDNA, Fungal/geneticsData Interpretation, StatisticalHumansMicrobiological Techniques/methodsMycoses/blood/diagnosis/microbiologyPolymerase Chain Reaction/methods/standardsReference StandardsReproducibility of ResultsRetrospective StudiesSpectrometry, Mass, Electrospray Ionization/methods/standardsSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods/standardsYeasts/classification/genetics/isolation & purification
Identifiers
PMID: 21576270
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Article (Published version) (536 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Research group Analyse génomique et fonctionnelle du staphylocoque doré (604)
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(ISO format)
KALETA, Erin J et al. Comparative analysis of PCR-electrospray ionization/mass spectrometry (MS) and MALDI-TOF/MS for the identification of bacteria and yeast from positive blood culture bottles. In: Clinical Chemistry, 2011, vol. 57, n° 7, p. 1057-67. https://archive-ouverte.unige.ch/unige:25447

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Deposited on : 2013-01-14

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