en
Scientific article
Meta-analysis
English

Comparative analysis of PCR-electrospray ionization/mass spectrometry (MS) and MALDI-TOF/MS for the identification of bacteria and yeast from positive blood culture bottles

Published inClinical chemistry, vol. 57, no. 7, p. 1057-1067
Publication date2011
Abstract

Emerging technologies for rapid identification of microbes demonstrate a shift from traditional biochemical and molecular testing algorithms toward methods using mass spectrometry (MS) for the semiquantitative analysis of microbial proteins and genetic elements. This study was performed to assess the diagnostic accuracy of 2 such technologies, PCR-electrospray ionization (ESI)/MS and MALDI-TOF/MS, with respect to phenotypic and biochemical profiling as a reference standard method. A positive challenge set of blood culture bottles was used to compare PCR-ESI/MS and MALDI-TOF/MS performance on a matched set of samples.

Keywords
  • Bacteremia/blood/diagnosis/microbiology
  • Bacteria/classification/genetics/isolation & purification
  • Base Sequence
  • DNA, Bacterial/genetics
  • DNA, Fungal/genetics
  • Data Interpretation, Statistical
  • Humans
  • Microbiological Techniques/methods
  • Mycoses/blood/diagnosis/microbiology
  • Polymerase Chain Reaction/methods/standards
  • Reference Standards
  • Reproducibility of Results
  • Retrospective Studies
  • Spectrometry, Mass, Electrospray Ionization/methods/standards
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods/standards
  • Yeasts/classification/genetics/isolation & purification
Citation (ISO format)
KALETA, Erin J et al. Comparative analysis of PCR-electrospray ionization/mass spectrometry (MS) and MALDI-TOF/MS for the identification of bacteria and yeast from positive blood culture bottles. In: Clinical chemistry, 2011, vol. 57, n° 7, p. 1057–1067. doi: 10.1373/clinchem.2011.161968
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Article (Published version)
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ISSN of the journal0009-9147
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