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Scientific article
Meta-analysis
English

Comparative analysis of PCR-electrospray ionization/mass spectrometry (MS) and MALDI-TOF/MS for the identification of bacteria and yeast from positive blood culture bottles

Contributors

Kaleta, Erin J; Clark, Andrew E; Cherkaoui, Abdessalam; Wysocki, Vicki H; Ingram, Elizabeth L; Schrenzel, Jacques; Wolk, Donna M

Published in

Clinical chemistry, vol. 57, no. 7, p. 1057-1067

Publication date

2011

Abstract

Emerging technologies for rapid identification of microbes demonstrate a shift from traditional biochemical and molecular testing algorithms toward methods using mass spectrometry (MS) for the semiquantitative analysis of microbial proteins and genetic elements. This study was performed to assess the diagnostic accuracy of 2 such technologies, PCR-electrospray ionization (ESI)/MS and MALDI-TOF/MS, with respect to phenotypic and biochemical profiling as a reference standard method. A positive challenge set of blood culture bottles was used to compare PCR-ESI/MS and MALDI-TOF/MS performance on a matched set of samples.

Keywords

  • Bacteremia/blood/diagnosis/microbiology
  • Bacteria/classification/genetics/isolation & purification
  • Base Sequence
  • DNA, Bacterial/genetics
  • DNA, Fungal/genetics
  • Data Interpretation, Statistical
  • Humans
  • Microbiological Techniques/methods
  • Mycoses/blood/diagnosis/microbiology
  • Polymerase Chain Reaction/methods/standards
  • Reference Standards
  • Reproducibility of Results
  • Retrospective Studies
  • Spectrometry, Mass, Electrospray Ionization/methods/standards
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods/standards
  • Yeasts/classification/genetics/isolation & purification

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

KALETA, Erin J et al. Comparative analysis of PCR-electrospray ionization/mass spectrometry (MS) and MALDI-TOF/MS for the identification of bacteria and yeast from positive blood culture bottles. In: Clinical chemistry, 2011, vol. 57, n° 7, p. 1057–1067. doi: 10.1373/clinchem.2011.161968

Main files (1)

Article (Published version)

Identifiers

Journal ISSN

0009-9147
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Technical informations

Creation

18/10/2012 14:06:00

First validation

18/10/2012 14:06:00

Update

13/01/2025 13:53:58

Status update

13/01/2025 13:53:58

Last indexation

13/01/2025 14:04:57
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