en
Scientific article
English

Correlation of daptomycin resistance in a clinical Staphylococcus aureus strain with increased cell wall teichoic acid production and D-alanylation

Published inAntimicrobial agents and chemotherapy, vol. 55, no. 8, p. 3922-3928
Publication date2011
Abstract

Cell wall thickening is a common feature among daptomycin-resistant Staphylococcus aureus strains. However, the mechanism(s) leading to this phenotype is unknown. We examined a number of cell wall synthesis pathway parameters in an isogenic strain set of S. aureus bloodstream isolates obtained from a patient with recalcitrant endocarditis who failed daptomycin therapy, including the initial daptomycin-susceptible parental strain (strain 616) and two daptomycin-resistant strains (strains 701 and 703) isolated during daptomycin therapy. Transmission electron microscopy demonstrated significantly thicker cell walls in the daptomycin-resistant strains than in the daptomycin-susceptible strain, a finding which was compatible with significant differences in dry cell weight of strain 616 versus strains 701 to 703 (P < 0.05). Results of detailed analysis of cell wall muropeptide composition, the degree of peptide side chain cross-linkage, and the amount of the peptidoglycan precursor, UDP-MurNAc-pentapeptide, were similar in the daptomycin-susceptible and daptomycin-resistant isolates. In contrast, the daptomycin-resistant strains contained less O-acetylated peptidoglycan. Importantly, both daptomycin-resistant strains synthesized significantly more wall teichoic acid (WTA) than the parental strain (P < 0.001). Moreover, the proportion of D-alanylated WTA species was substantially higher in the daptomycin-resistant strains than in the daptomycin-susceptible parental strain (P < 0.05 in comparing strain 616 versus strain 701). The latter phenotypic findings correlated with (i) enhanced tagA and dltA gene expression, respectively, and (ii) an increase in surface positive charge observed in the daptomycin-resistant versus daptomycin-susceptible isolates. Collectively, these data suggest that increases in WTA synthesis and the degree of its D-alanylation may play a major role in the daptomycin-resistant phenotype in some S. aureus strains.

Keywords
  • Alanine/metabolism
  • Anti-Bacterial Agents/analysis/pharmacology/therapeutic use
  • Bacterial Proteins/biosynthesis
  • Cell Wall/chemistry/metabolism
  • Daptomycin/analysis/pharmacology/therapeutic use
  • Drug Resistance, Bacterial
  • Endocarditis, Bacterial/microbiology
  • Humans
  • Lipoproteins/biosynthesis
  • Microbial Sensitivity Tests
  • Microscopy, Electron, Transmission
  • Peptidoglycan/biosynthesis
  • Staphylococcal Infections/drug therapy/microbiology
  • Staphylococcus aureus/drug effects/isolation & purification/metabolism
  • Teichoic Acids/biosynthesis
  • Uridine Diphosphate N-Acetylmuramic Acid/analogs & derivatives/biosynthesis
Citation (ISO format)
BERTSCHE, Ute et al. Correlation of daptomycin resistance in a clinical Staphylococcus aureus strain with increased cell wall teichoic acid production and D-alanylation. In: Antimicrobial agents and chemotherapy, 2011, vol. 55, n° 8, p. 3922–3928. doi: 10.1128/AAC.01226-10
Main files (1)
Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal0066-4804
471views
0downloads

Technical informations

Creation10/18/2012 2:00:00 PM
First validation10/18/2012 2:00:00 PM
Update time03/14/2023 7:56:33 PM
Status update03/14/2023 7:56:33 PM
Last indexation01/16/2024 12:54:32 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack