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Title

Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for rapid identification of Beta-hemolytic streptococci

Authors
Fernandez, José
Schorderet, Didier
Published in Journal of Clinical Microbiology. 2011, vol. 49, no. 8, p. 3004-5
Abstract This study was undertaken to evaluate matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for the rapid identification of beta-hemolytic streptococci. We compared Bruker Biotyper 2.0 with Vitek2 coupled to the agglutination test. MALDI-TOF MS analysis of 386 beta-hemolytic streptococcal isolates yielded high-confidence identification to the species level for all 386 isolates. The Vitek2 gave high-confidence identification to the species level for 88% of Streptococcus agalactiae isolates (n = 269/306), 92% of Streptococcus pyogenes isolates (n = 48/52), and 39% of isolates of Streptococcus dysgalactiae serogroups C and G (n = 11/28).
Keywords Bacteriological Techniques/methodsHumansSensitivity and SpecificitySpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methodsStreptococcal Infections/diagnosis/microbiologyStreptococcus/chemistry/classification/isolation & purification
Identifiers
PMID: 21697322
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Structures
Research group Analyse génomique et fonctionnelle du staphylocoque doré (604)
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CHERKAOUI, Abdessalam et al. Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for rapid identification of Beta-hemolytic streptococci. In: Journal of Clinical Microbiology, 2011, vol. 49, n° 8, p. 3004-5. https://archive-ouverte.unige.ch/unige:25443

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Deposited on : 2013-01-14

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